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Culturable bacteria in the glacial meltwater in the ablation zones of glacier at high altitude (6,350 m) on Mt Everest were isolated and identified by 16S rRNA amplification and sequencing. The obtained sequences revealed the presence of members of alpha, beta, and gamma-Proteobacteria, Actinobacteria, and Firmicutes, with the Actinobacteria dominant in the studied habitat. All 16S rRNA sequences were similar to previously determined sequences, ranging from 97 to 99% identical values. The strains isolated from meltwater were distinctly different from those recovered from a cryoconite hole and under glacier habitat. The majority of the isolates‘ nearest neighbors were from the permafrost, dust, soil, plant, and aqueous environments. The Biolog bioassay and growth test under different temperatures suggested that the culturable bacteria in glacial meltwater could be divided into three categories in terms of their survival strategies: Group I sensitive to temperature change but versatile in utilization of carbon substrates (capable of utilization of about 70% of the Biolog carbon substrates); Group II tolerant to variable temperature and less capable of carbon utilization (less than half of the Biolog carbon species can be used); Group III slow in growth and weak in carbon utilization (only a few Biolog carbon substrates can be used).