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DOI10.1111/j.1574-6968.2006.00477.x
Microbial community structure in moraine lakes and glacial meltwaters, Mount Everest
Liu, Yongqin; Yao, Tandong; Jiao, Nianzhi; Kang, Shichang; Zeng, Yonghui; Huang, Sijun
发表日期2006
ISSN0378-1097
EISSN1574-6968
起始页码98
结束页码105
卷号265期号:1页码:98-105
英文摘要The bacterial diversity and abundance in two moraine lakes and two glacial meltwaters (5140, 5152, 5800 and 6350 m above sea level, respectively) in the remote Mount Everest region were examined through 16S rRNA gene clone library and flow cytometry approaches. In total, 247 clones were screened by RFLP and 60 16S rRNA gene sequences were obtained, belonging to the following groups: Proteobacteria (8% alpha subdivision, 21% beta subdivision, and 1% gamma subdivision), Cytophaga-Flavobacteria-Bacteroides (CFB) (54%), Actinobacteria (4%), Planctomycetes (2%), Verrucomicrobia (2%), Fibrobacteres (1%) and Eukaryotic chroloplast (3%), respectively. The high dominance of CFB distinguished the Mount Everest waters from other mountain lakes. The highest bacterial abundance and diversity occurred in the open moraine lake at 5152 m, and the lowest in the glacial meltwater at 6350 m. Low temperature at high altitude is considered to be critical for component dominancy. At the same altitude, nutrient availability plays a role in regulating population structure. Our results also show that the bacteria in Mount Everest may be derived from different sources.
关键词RIBOSOMAL DATABASE PROJECTRNA GENE-SEQUENCESMALAN ICE COREBACTERIAL DIVERSITYTIBETAN PLATEAUCLIMATE-CHANGEWINTER COVER16S RDNARDP-IISP-NOV
英文关键词Mount Everest; moraine lake; glacial meltwater; bacterial diversity and abundance
语种英语
WOS研究方向Microbiology
WOS类目Microbiology
WOS记录号WOS:000241893800014
来源期刊FEMS MICROBIOLOGY LETTERS
来源机构中国科学院青藏高原研究所
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/443
推荐引用方式
GB/T 7714
Liu, Yongqin,Yao, Tandong,Jiao, Nianzhi,et al. Microbial community structure in moraine lakes and glacial meltwaters, Mount Everest[J]. 中国科学院青藏高原研究所,2006,265(1):98-105.
APA Liu, Yongqin,Yao, Tandong,Jiao, Nianzhi,Kang, Shichang,Zeng, Yonghui,&Huang, Sijun.(2006).Microbial community structure in moraine lakes and glacial meltwaters, Mount Everest.FEMS MICROBIOLOGY LETTERS,265(1),98-105.
MLA Liu, Yongqin,et al."Microbial community structure in moraine lakes and glacial meltwaters, Mount Everest".FEMS MICROBIOLOGY LETTERS 265.1(2006):98-105.
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