气候变化领域集成服务门户(CCPortal): De novo transcriptome sequencing and annotation of the Antarctic polychaete Microspio moorei (Spionidae) with its characterization of the heat stress-related proteins (HSP, SOD & CAT)

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DOI	10.1016/j.margen.2024.101085
	De novo transcriptome sequencing and annotation of the Antarctic polychaete Microspio moorei (Spionidae) with its characterization of the heat stress-related proteins (HSP, SOD & CAT)
	Fonseca-Gonzalez, Idalyd; Velasquez-Agudelo, Esteban; Londono-Mesa, Mario H.; Alvarez, Javier C.
发表日期	2024
ISSN	1874-7787
EISSN	1876-7478
起始页码	73
卷号	73
英文摘要	We present a de novo transcriptome assembly for the non-model Antarctic polychaete worm Microspio moorei (Spionidae) collected during Antarctic field expedition in Fildes Bay, King George Island, Antarctic Peninsula, in 2017. Here, we report the first transcriptome reference array for Microspio spp. The gene sequences of the spionid worm were annotated from a wide range of functions (i.e., biological, and metabolic processes, catalytic processes, and catalytic activity). HSP70, HSP90 SOD and CAT families were compared to reported annelid transcriptomes and proteomes. The phylogenetic analysis using COI, 16S, and 18S markers effectively clusters the species within the family. However, it also casts uncertainty on the monophyletic nature of the Microspio genera, indicating the necessity for additional data and potentially requiring a reevaluation of its grouping. Within these protein families, 3D model software was used to create one representative of their protein structures. Structural predictions were compared with related reported annelids living at different temperatures and a human X-ray reference. We found structural differences (RMSE >1.8) between the human HSP proteins but no significant differences between the polychaete-predicted proteins (RMSE <1.2). These results encourage further research of heat stress-related proteins, the development of genetic markers for climate change-induced temperature stress, and the study of the underlying mechanisms of the heat response. Moreover, these results motivate the extension of these findings to congeneric species.
英文关键词	Climate change; Ocean warming; RNA-seq; Antarctic invertebrates; Heat-stress proteins; Protein structures
语种	英语
WOS研究方向	Genetics & Heredity ; Marine & Freshwater Biology
WOS类目	Genetics & Heredity ; Marine & Freshwater Biology
WOS记录号	WOS:001177661300001
来源期刊	MARINE GENOMICS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/309816
作者单位	Universidad de Antioquia; Universidad de Antioquia; Universidad EAFIT
推荐引用方式 GB/T 7714	Fonseca-Gonzalez, Idalyd,Velasquez-Agudelo, Esteban,Londono-Mesa, Mario H.,et al. De novo transcriptome sequencing and annotation of the Antarctic polychaete Microspio moorei (Spionidae) with its characterization of the heat stress-related proteins (HSP, SOD & CAT)[J],2024,73.
APA	Fonseca-Gonzalez, Idalyd,Velasquez-Agudelo, Esteban,Londono-Mesa, Mario H.,&Alvarez, Javier C..(2024).De novo transcriptome sequencing and annotation of the Antarctic polychaete Microspio moorei (Spionidae) with its characterization of the heat stress-related proteins (HSP, SOD & CAT).MARINE GENOMICS,73.
MLA	Fonseca-Gonzalez, Idalyd,et al."De novo transcriptome sequencing and annotation of the Antarctic polychaete Microspio moorei (Spionidae) with its characterization of the heat stress-related proteins (HSP, SOD & CAT)".MARINE GENOMICS 73(2024).