气候变化领域集成服务门户(CCPortal): Climate change can impair bacterial pathogen defences in sablefish via hypoxia-mediated effects on adaptive immunity

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DOI	10.1016/j.dci.2024.105161
	Climate change can impair bacterial pathogen defences in sablefish via hypoxia-mediated effects on adaptive immunity
	Leeuwis, Robine H. J.; Hall, Jennifer R.; Zanuzzo, Fabio S.; Smith, Nicole; Clow, Kathy A.; Kumar, Surendra; Vasquez, Ignacio; Goetz, Frederick W.; Johnson, Stewart C.; Rise, Matthew L.; Santander, Javier; Gamperl, A. Kurt
发表日期	2024
ISSN	0145-305X
EISSN	1879-0089
起始页码	156
卷号	156
英文摘要	Low -oxygen levels (hypoxia) in aquatic habitats are becoming more common because of global warming and eutrophication. However, the effects on the health/disease status of fishes, the world's largest group of vertebrates, are unclear. Therefore, we assessed how long-term hypoxia affected the immune function of sablefish, an ecologically and economically important North Pacific species, including the response to a formalin-killed Aeromonas salmonicida bacterin. Sablefish were held at normoxia or hypoxia (100% or 40% air saturated seawater, respectively) for 6-16 weeks, while we measured a diverse array of immunological traits. Given that the sablefish is a non -model organism, this involved the development of a species -specific methodological toolbox comprised of qPCR primers for 16 key immune genes, assays for blood antibacterial defences, the assessment of blood immunoglobulin (IgM) levels with ELISA, and flow cytometry and confocal microscopy techniques. We show that innate immune parameters were typically elevated in response to the bacterial antigens, but were not substantially affected by hypoxia. In contrast, hypoxia completely prevented the similar to 1.5 -fold increase in blood IgM level that was observed under normoxic conditions following bacterin exposure, implying a serious impairment of adaptive immunity. Since the sablefish is naturally hypoxia tolerant, our results demonstrate that climate change -related deoxygenation may be a serious threat to the immune competency of fishes.
英文关键词	Sablefish ( Anoplopoma fimbria ); Chronic hypoxia; Innate immunity; Adaptive immunity; Bacterial pathogen; Climate change
语种	英语
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS类目	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS记录号	WOS:001235309300001
来源期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/309792
作者单位	Memorial University Newfoundland; Memorial University Newfoundland; University of Wisconsin System; University of Wisconsin Milwaukee; Fisheries & Oceans Canada; Norwegian University of Science & Technology (NTNU); Fisheries & Oceans Canada
推荐引用方式 GB/T 7714	Leeuwis, Robine H. J.,Hall, Jennifer R.,Zanuzzo, Fabio S.,et al. Climate change can impair bacterial pathogen defences in sablefish via hypoxia-mediated effects on adaptive immunity[J],2024,156.
APA	Leeuwis, Robine H. J..,Hall, Jennifer R..,Zanuzzo, Fabio S..,Smith, Nicole.,Clow, Kathy A..,...&Gamperl, A. Kurt.(2024).Climate change can impair bacterial pathogen defences in sablefish via hypoxia-mediated effects on adaptive immunity.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,156.
MLA	Leeuwis, Robine H. J.,et al."Climate change can impair bacterial pathogen defences in sablefish via hypoxia-mediated effects on adaptive immunity".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 156(2024).