气候变化领域集成服务门户(CCPortal): Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet

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DOI	10.7554/eLife.86169
	Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet
	de la Fuente, Carla; Grondin, Alexandre; Sine, Bassirou; Debieu, Marilyne; Belin, Christophe; Hajjarpoor, Amir; Atkinson, Jonathan A.; Passot, Sixtine; Salson, Marine; Orjuela, Julie; Tranchant-Dubreuil, Christine; Brossier, Jean-Remy; Steffen, Maxime; Morgado, Charlotte; Dinh, Hang Ngan; Pandey, Bipin K.; Darmau, Julie; Champion, Antony; Petitot, Anne-Sophie; Barrachina, Celia; Pratlong, Marine; Mounier, Thibault; Nakombo-Gbassault, Princia; Gantet, Pascal; Gangashetty, Prakash; Guedon, Yann; Vadez, Vincent; Reichheld, Jean-Philippe; Bennett, Malcolm J.; Kane, Ndjido Ardo; Guyomarc'h, Soazig; Wells, Darren M.; Vigouroux, Yves; Laplaze, Laurent
发表日期	2024
ISSN	2050-084X
起始页码	12
卷号	12
英文摘要	Seedling root traits impact plant establishment under challenging environments. Pearl millet is one of the most heat and drought tolerant cereal crops that provides a vital food source across the sub-Saharan Sahel region. Pearl millet's early root system features a single fast-growing primary root which we hypothesize is an adaptation to the Sahelian climate. Using crop modeling, we demonstrate that early drought stress is an important constraint in agrosystems in the Sahel where pearl millet was domesticated. Furthermore, we show that increased pearl millet primary root growth is correlated with increased early water stress tolerance in field conditions. Genetics including genome-wide association study and quantitative trait loci (QTL) approaches identify genomic regions controlling this key root trait. Combining gene expression data, re-sequencing and re-annotation of one of these genomic regions identified a glutaredoxin-encoding gene PgGRXC9 as the candidate stress resilience root growth regulator. Functional characterization of its closest Arabidopsis homolog AtROXY19 revealed a novel role for this glutaredoxin (GRX) gene clade in regulating cell elongation. In summary, our study suggests a conserved function for GRX genes in conferring root cell elongation and enhancing resilience of pearl millet to its Sahelian environment. eLife assessment This is an important paper that combines methods ranging from agronomy and plant breeding to Arabidopsis functional genetics, to argue that polymorphism in a single gene affects crop yield in pearl millet by affecting root cell elongation and drought stress resilience in a poorly studied crop. The overall argument is plausible but whether the solid evidence generated with Arabidopsis experiments can be extended to pearl millet itself is unclear. eLife digest Pearl millet is a staple food for over 90 million people living in regions of Africa and India that typically experience high temperatures and little rainfall. It was domesticated about 4,500 years ago in the Sahel region of West Africa and is one of the most heat and drought tolerant cereal crops worldwide. In most plants, organs known as roots absorb water and essential nutrients from the soil. Young pearl millet plants develop a fast-growing primary root, but it is unclear how this unique feature helps the crop to grow in hot and dry conditions. Using weather data collected from the Sahel over a 20-year period, Fuente, Grondin et al. predicted by modelling that early drought stress is the major factor limiting pearl millet growth and yield in this region. Field experiments found that plants with primary roots that grow faster within soil were better at tolerating early drought than those with slower growing roots. Further work using genetic approaches revealed that a gene known as PgGRXC9 promotes the growth of the primary root. To better understand how this gene works, the team examined a very similar gene in a well-studied model plant known as Arabidopsis. This suggested that PgGRXC9 helps the primary root to grow by stimulating cell elongation within the root. Since it is well adapted to dry conditions, pearl millet is expected to play an important role in helping agriculture adjust to climate change. The findings of Fuente, Grondin et al. may be used by plant breeders to create more resilient and productive varieties of pearl millet.
英文关键词	pearl millet; redox; cell elongation; GWAS; stress pattern; root meristem; A. thaliana
语种	英语
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:001159600800001
来源期刊	ELIFE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/306823
作者单位	Universite de Montpellier; Institut de Recherche pour le Developpement (IRD); CIRAD; Universite Perpignan Via Domitia; University of Nottingham; Universite de Montpellier; CGIAR; International Crops Research Institute for the Semi-Arid-Tropics (ICRISAT); Universite de Montpellier; Institut Agro; CIRAD; INRAE; Centre National de la Recherche Scientifique (CNRS)
推荐引用方式 GB/T 7714	de la Fuente, Carla,Grondin, Alexandre,Sine, Bassirou,et al. Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet[J],2024,12.
APA	de la Fuente, Carla.,Grondin, Alexandre.,Sine, Bassirou.,Debieu, Marilyne.,Belin, Christophe.,...&Laplaze, Laurent.(2024).Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet.ELIFE,12.
MLA	de la Fuente, Carla,et al."Glutaredoxin regulation of primary root growth is associated with early drought stress tolerance in pearl millet".ELIFE 12(2024).