气候变化领域集成服务门户(CCPortal): Temperature regulates sex determination and growth in the paralichthid flatfish California halibut

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DOI	10.1002/jez.2823
	Temperature regulates sex determination and growth in the paralichthid flatfish California halibut
	Macnamara, Ruairi; Mankiewicz, Jamie L.; Salger, Scott A.; Stuart, Kevin; Borski, Russell J.; Godwin, John; Drawbridge, Mark
发表日期	2024
ISSN	2471-5638
EISSN	2471-5646
英文摘要	California halibut (Paralichthys californicus) is a candidate species for aquaculture and stock enhancement. These applications rely on sex control, either to maximize the production of faster growing females or to match sex ratios in the wild. Other paralichthids exhibit temperature-dependent sex determination (TSD), but the presence and pattern of TSD is not well defined in California halibut. Juvenile California halibut were cultured at three distinct temperatures (15 degrees C, 19 degrees C, and 23 degrees C) through the developmental period presumed to be thermosensitive based on findings from congeners. Sex ratios were quantified in each treatment using phenotypic sex identification techniques applied early (molecular biomarkers; 51-100 mm total length [TL]) and late (visual examination of the gonads; >= 100 mm TL) in the juvenile phase. Both techniques indicated similar sex determination trends at each temperature, with overall sex ratios assessed as 49.9% male at 15 degrees C, 74.5% male at 19 degrees C, and 98.2% male at 23 degrees C. Growth rates were highest at 23 degrees C and lowest at 15 degrees C, with intrinsically fast- and slow-growing individuals at all temperatures. At 15 degrees C and 19 degrees C, females comprised a higher proportion among the fast growers than they did among the slow growers. These data show that California halibut exhibit TSD, with temperatures of 19 degrees C and 23 degrees C masculinizing fish while 15 degrees C appears to produce a 1:1 sex ratio. This study will help optimize sex ratios and growth in hatcheries through thermal manipulation. Furthermore, the developed biomolecular tools and identified temperature thresholds will be important in future work to understand the influence of global warming on wild population demographics. Phenotypic sex was identified using molecular biomarkers and visual examination in California halibut exposed to 15 degrees C, 19 degrees C, and 23 degrees C during early development. An equal sex ratio occurred at 15 degrees C and masculinization was induced at the higher temperatures, thus confirming temperature-dependent sex determination in this species. image California halibut exhibit temperature-dependent sex determination. Rearing at 15 degrees C produced an equal sex ratio, while exposure to higher temperatures (19 degrees C and 23 degrees C) caused masculinization. Expression of sex-related genes (amh, cyp19a1a, foxl2) can be used to identify phenotypic sex.
英文关键词	anti-m & uuml;llerian hormone; climate change; molecular biomarkers; sex identification; sex ratio; temperature-dependent sex determination; thermal physiology
语种	英语
WOS研究方向	Zoology
WOS类目	Zoology
WOS记录号	WOS:001214610900001
来源期刊	JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/306064
作者单位	North Carolina State University
推荐引用方式 GB/T 7714	Macnamara, Ruairi,Mankiewicz, Jamie L.,Salger, Scott A.,et al. Temperature regulates sex determination and growth in the paralichthid flatfish California halibut[J],2024.
APA	Macnamara, Ruairi.,Mankiewicz, Jamie L..,Salger, Scott A..,Stuart, Kevin.,Borski, Russell J..,...&Drawbridge, Mark.(2024).Temperature regulates sex determination and growth in the paralichthid flatfish California halibut.JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY.
MLA	Macnamara, Ruairi,et al."Temperature regulates sex determination and growth in the paralichthid flatfish California halibut".JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY (2024).