气候变化领域集成服务门户(CCPortal): Environmental DNA as a detection tool for small-bodied, cryptic, threatened fish in a highly turbid freshwater lake system

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DOI	10.1071/WR23027
	Environmental DNA as a detection tool for small-bodied, cryptic, threatened fish in a highly turbid freshwater lake system
	Stoessel, D. J.; Raadik, T. A.; Adams, M.; Shelley, J. J.; Hately, T. J.; Iervasi, D.; Rose, P.; Russell, A.; Murphy, N.; Stow, Adam
发表日期	2024
ISSN	1035-3712
EISSN	1448-5494
起始页码	51
结束页码	1
卷号	51 期号:1
英文摘要	ContextWhere conservation efforts are undertaken to decrease downward trends in fish populations, comparatively few resources are directed to small-bodied cryptic species. The true extent of the decline of many of these species is therefore often unknown. Where surveys have occurred, they are frequently limited by budget and hence effort. Consequently, there is a risk that rare species may not be physically captured despite their presence at a site. Such an outcome has dire consequences for the conservation of remnant populations of threatened fish. To counter possible false negative detections, environmental DNA is increasingly being used in conjunction with, or as a precursor to, physical surveys. The Southern Purple-spotted Gudgeon (Mogurnda adspersa) is a small, threatened freshwater fish native to Australia. Recent surveys captured M. adspersa in two highly turbid waterbodies in north-central Victoria. This capture represented the first detection of the species in the state in more than 20 years. Because these waterbodies are part of a network of hydrologically connected systems, it was suspected that the species likely had a broader distribution in the region.AimsTo develop a probe-based eDNA assay for M. adspersa and compare its sensitivity against a physical sampling program.MethodsDetection (presence/absence) between eDNA and traditional surveys was compared across multiple sites.Key resultseDNA presents an effective tool for determining the presence of M. adspersa. The eDNA survey demonstrated significant clustering of eDNA detections towards the outlets of lakes sampled, suggesting concentrated eDNA at this point, or that, due to the channels being shallower, the eDNA of resident individuals may be less diluted.ConclusionsBased on these results, future survey of rare, cryptic species in highly turbid lake systems should in the first instance include a broad scale eDNA survey, with sampling concentrated at outlet channels.ImplicationsThe likely most cost-effective approach to determining the presence/absence of rare species in lake systems is the collection of eDNA samples at outlet channels. Comparatively few resources are devoted to the detection of rare small-bodied cryptic fish species. The extent of decline of many of these species is therefore often unknown. We found significant clustering of eDNA of such a species towards the outlets of lakes, suggesting that sampling for detection (presence/absence) in such environments should be undertaken at outlet channels to minimise cost and maximise opportunities to detect rare aquatic species. Photograph by Doug Gimesy, Department of Energy, Environment and Climate Change.
英文关键词	conservation; conservation management; endangered species; geographical range; native; population distribution
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Zoology
WOS类目	Ecology ; Zoology
WOS记录号	WOS:001141199300040
来源期刊	WILDLIFE RESEARCH
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/305779
作者单位	Arthur Rylah Institute for Environmental Research (ARI); South Australian Museum; University of Adelaide; La Trobe University; North Central Catchment Management Authority
推荐引用方式 GB/T 7714	Stoessel, D. J.,Raadik, T. A.,Adams, M.,et al. Environmental DNA as a detection tool for small-bodied, cryptic, threatened fish in a highly turbid freshwater lake system[J],2024,51(1).
APA	Stoessel, D. J..,Raadik, T. A..,Adams, M..,Shelley, J. J..,Hately, T. J..,...&Stow, Adam.(2024).Environmental DNA as a detection tool for small-bodied, cryptic, threatened fish in a highly turbid freshwater lake system.WILDLIFE RESEARCH,51(1).
MLA	Stoessel, D. J.,et al."Environmental DNA as a detection tool for small-bodied, cryptic, threatened fish in a highly turbid freshwater lake system".WILDLIFE RESEARCH 51.1(2024).