气候变化领域集成服务门户(CCPortal): Chamber #8 - a holistic approach of high-throughput non-destructive assessment of plant roots

CCPortal

DOI	10.3389/fpls.2023.1269005
	Chamber #8 - a holistic approach of high-throughput non-destructive assessment of plant roots
	Claussen, Joelle; Wittenberg, Thomas; Uhlmann, Norman; Gerth, Stefan
发表日期	2024
ISSN	1664-462X
起始页码	14
卷号	14
英文摘要	IntroductionIn the past years, it has been observed that the breeding of plants has become more challenging, as the visible difference in phenotypic data is much smaller than decades ago. With the ongoing climate change, it is necessary to breed crops that can cope with shifting climatic conditions. To select good breeding candidates for the future, phenotypic experiments can be conducted under climate-controlled conditions. Above-ground traits can be assessed with different optical sensors, but for the root growth, access to non-destructively measured traits is much more challenging. Even though MRI or CT imaging techniques have been established in the past years, they rely on an adequate infrastructure for the automatic handling of the pots as well as the controlled climate.MethodsTo address both challenges simultaneously, the non-destructive imaging of plant roots combined with a highly automated and standardized mid-throughput approach, we developed a workflow and an integrated scanning facility to study root growth. Our chamber #8 contains a climate chamber, a material flow control, an irrigation system, an X-ray system, a database for automatic data collection, and post-processing. The goals of this approach are to reduce the human interaction with the various components of the facility to a minimum on one hand, and to automate and standardize the complete process from plant care via measurements to root trait calculation on the other. The user receives standardized phenotypic traits and properties that were collected objectively.ResultsThe proposed holistic approach allows us to study root growth of plants in a field-like substrate non-destructively over a defined period and to calculate phenotypic traits of root architecture. For different crops, genotypic differences can be observed in response to climatic conditions which have already been applied to a wide variety of root structures, such as potatoes, cassava, or corn.DiscussionIt enables breeders and scientists non-destructive access to root traits. Additionally, due to the non-destructive nature of X-ray computed tomography, the analysis of time series for root growing experiments is possible and enables the observation of kinetic traits. Furthermore, using this automation scheme for simultaneously controlled plant breeding and non-destructive testing reduces the involvement of human resources.
英文关键词	phenotyping; x-ray; CT; non-destructive testing; roots; data management
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:001142924900001
来源期刊	FRONTIERS IN PLANT SCIENCE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/305520
作者单位	Fraunhofer Gesellschaft; University of Erlangen Nuremberg
推荐引用方式 GB/T 7714	Claussen, Joelle,Wittenberg, Thomas,Uhlmann, Norman,et al. Chamber #8 - a holistic approach of high-throughput non-destructive assessment of plant roots[J],2024,14.
APA	Claussen, Joelle,Wittenberg, Thomas,Uhlmann, Norman,&Gerth, Stefan.(2024).Chamber #8 - a holistic approach of high-throughput non-destructive assessment of plant roots.FRONTIERS IN PLANT SCIENCE,14.
MLA	Claussen, Joelle,et al."Chamber #8 - a holistic approach of high-throughput non-destructive assessment of plant roots".FRONTIERS IN PLANT SCIENCE 14(2024).