气候变化领域集成服务门户(CCPortal): Multi-trait efficiency and interactivity of bacterial consortia used to enhance plant performance under water stress conditions

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DOI	10.1016/j.micres.2024.127610
	Multi-trait efficiency and interactivity of bacterial consortia used to enhance plant performance under water stress conditions
	Thomas-Barry, Gem; St Martin, Chaney; Ramsubhag, Adesh; Eudoxie, Gaius; Miller, Judy Rouse
发表日期	2024
ISSN	0944-5013
EISSN	1618-0623
起始页码	281
卷号	281
英文摘要	Water stress is a major limiting factor for agricultural production under current and projected climate change scenarios. As a sustainable strategy, plant growth-promoting bacterial consortia have been used to reduce plant water stress. However, few studies have examined the effects of stress on multi-trait efficiency and interactivity of bacterial species. In this study, we used several in-vitro experiments, plant assays and greenhouse trials to investigate the effects of stress and bacterial consortia on 1-aminocyclopropane-1-carboxylic acid deaminase (ACCD) activities, indole-3-acetic acid (IAA) production and plant growth-promoting traits (Phosphate-solubilization, starch hydrolysis, siderophores and ammonium production). We further assessed biofilm formation and the chemotactic behaviour in response to ACC. A total of fifteen ACCD rhizobacteria with multiple growthpromoting traits from the dominant plant species from the hyperseasonal Aripo Savannas were screened in this study. Five of the isolates were further analyzed based on their ACCD activities and were tested in single and dual consortium to assess their abilities in promoting growth under simulated drought stress (-0.35 MPa) and chemically induced ACC conditions (0.03 mM). Our findings showed that bacteria which produce high concentrations of IAA affected the isolates' ability to promote growth under stress, irrespective of microbial combination with ACCD activity above the minimal threshold of 20 nmol alpha-ketobutyrate mg-1 h-1. Biofilm production with co-culture interaction varied greatly across treatments, however, the general trend showed an increase in biofilm under stress induce conditions. The best performing co-culture, UWIGT-83 and UWIGT-120 (Burkholderia sp.) showed enhanced growth in germination assays and in greenhouse trials with Capsicum chinense (Moruga red hot peppers) under drought stress, when compared to non-inoculated treatments. The findings highlight the importance of testing interactivity of bacterial species with multiple growth promoting traits under stress conditions; and proposed the use of ACC growth media as a novel biofilm screening method for selecting potential stress plant growth-promoting bacteria. Better screening strategies for appropriate plant growthpromoting bacteria may narrow the inconsistency observed between laboratory and field trials.
英文关键词	ACC-deaminase; Indole-3-acetic acid; Rhizobacteria; Biofilm; Water -stress
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:001175289800001
来源期刊	MICROBIOLOGICAL RESEARCH
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/305279
作者单位	University West Indies Mona Jamaica; University West Indies Saint Augustine; University West Indies Mona Jamaica; University West Indies Saint Augustine
推荐引用方式 GB/T 7714	Thomas-Barry, Gem,St Martin, Chaney,Ramsubhag, Adesh,et al. Multi-trait efficiency and interactivity of bacterial consortia used to enhance plant performance under water stress conditions[J],2024,281.
APA	Thomas-Barry, Gem,St Martin, Chaney,Ramsubhag, Adesh,Eudoxie, Gaius,&Miller, Judy Rouse.(2024).Multi-trait efficiency and interactivity of bacterial consortia used to enhance plant performance under water stress conditions.MICROBIOLOGICAL RESEARCH,281.
MLA	Thomas-Barry, Gem,et al."Multi-trait efficiency and interactivity of bacterial consortia used to enhance plant performance under water stress conditions".MICROBIOLOGICAL RESEARCH 281(2024).