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| DOI | 10.1016/j.scitotenv.2024.172318 |
| Comprehensive analysis of histophysiology, transcriptome and metabolome tolerance mechanisms in black porgy ( Acanthopagrus schlegelii ) under low temperature stress | |
| Wang, Yue; Shen, Mingjun; Xu, Guangping; Yu, Han; Jia, Chaofeng; Zhu, Fei; Meng, Qian; Xu, Dafeng; Du, Shuran; Zhang, Dianchang; Zhang, Zhiwei | |
| 发表日期 | 2024 |
| ISSN | 0048-9697 |
| EISSN | 1879-1026 |
| 起始页码 | 927 |
| 卷号 | 927 |
| 英文摘要 | Low temperature stress has adverse effects on fish growth and reproduction, causing huge economic losses to the aquaculture industry. Especially, black porgy ( Acanthopagrus schlegelii ) farming industry in north of Yangtze River has been severely affected by low temperature for a long time. To explore the tolerance mechanism of black porgy to low temperature stress, the experiment was designed. The liver and gill tissues of black porgy were taken from the water temperature point of 15 degrees C (control group named as CG), 3.8 degrees C (cold sensitive group named as CS) and 2.8 degrees C (cold tolerant group named as CT) with a cooling rate of 3 degrees C/d from 15 degrees C for histophysiology, transcriptomics and metabolomics analysis. After cold stress, the histological results showed that the nucleus of the black porgy liver tissue appeared swelling, the cell arrangement was disordered; meanwhile the gill lamellae were twisted and broken, the epidermis was detached and aneurysm appeared. In addition, the expression of antioxidant, glucose metabolism and immune -related enzymes in the liver and gill of black porgy also changed significantly after low temperature stress. By analyzing the transcriptome and metabolome dates of black porgy liver, 3474 differentially expressed genes (DEGs) and 689 differentially expressed metabolites (DEMs) involved in low temperature stress were identified, respectively. The results of the transcriptome and metabolome combined analysis showed that individuals in the CS group mainly supplied energy to the body through lipid metabolism and amino acid metabolism, and meanwhile the apoptosis pathway was activated. While, individuals in the CT group mainly through glucose metabolism and steroid hormone biosynthesis to supply energy for the body. The validation results of qPCR on eight functional genes further demonstrated the reliability of RNA-Seq data. In summary, the results provide molecular information about adaptation to climate change and genetic selection of black porgy. |
| 英文关键词 | Black porgy(Acanthopagrusschlegelii); Low temperature stress; Histophysiology; Transcriptomics; Metabolomics |
| 语种 | 英语 |
| WOS研究方向 | Environmental Sciences & Ecology |
| WOS类目 | Environmental Sciences |
| WOS记录号 | WOS:001230373800001 |
| 来源期刊 | SCIENCE OF THE TOTAL ENVIRONMENT
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| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/304788 |
| 作者单位 | Shanghai Ocean University; Chinese Academy of Fishery Sciences; South China Sea Fisheries Research Institute, CAFS |
| 推荐引用方式 GB/T 7714 | Wang, Yue,Shen, Mingjun,Xu, Guangping,et al. Comprehensive analysis of histophysiology, transcriptome and metabolome tolerance mechanisms in black porgy ( Acanthopagrus schlegelii ) under low temperature stress[J],2024,927. |
| APA | Wang, Yue.,Shen, Mingjun.,Xu, Guangping.,Yu, Han.,Jia, Chaofeng.,...&Zhang, Zhiwei.(2024).Comprehensive analysis of histophysiology, transcriptome and metabolome tolerance mechanisms in black porgy ( Acanthopagrus schlegelii ) under low temperature stress.SCIENCE OF THE TOTAL ENVIRONMENT,927. |
| MLA | Wang, Yue,et al."Comprehensive analysis of histophysiology, transcriptome and metabolome tolerance mechanisms in black porgy ( Acanthopagrus schlegelii ) under low temperature stress".SCIENCE OF THE TOTAL ENVIRONMENT 927(2024). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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