气候变化领域集成服务门户(CCPortal): Comparative analysis of phytochemical profile and antioxidant and anti-inflammatory activity of four Gentiana species from the Qinghai-Tibet Plateau

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DOI	10.1016/j.jep.2024.117926
	Comparative analysis of phytochemical profile and antioxidant and anti-inflammatory activity of four Gentiana species from the Qinghai-Tibet Plateau
	Wang, Junjun; Liu, Ruimin; Zhang, Jianguang; Su, Hongna; Yang, Qing; Wulu, Jiansang; Li, Juan; Zhang, Zhifeng; Lv, Zhi
发表日期	2024
ISSN	0378-8741
EISSN	1872-7573
起始页码	326
卷号	326
英文摘要	Ethnopharmacological relevance: Gentiana species, known as the traditional Tibetan medicine Bangjian, have been integral to clinical practice for millennia. Despite their longstanding use, our understanding of the variation in chemical constituents and bioactive effects among different species is limited. Aim of the study: In the present study, we aimed to assess the differences in chemical profiles and bioactivities among four Gentiana species (G. veitchiorum, G. trichotoma, G. crassuloides, and G. squarrosa) and explore potential bioactive markers. Materials and methods: The chemical composition of the four Gentiana species was analyzed using UPLC-QEOrbitrap-MS. The antioxidant activity of the extracts was compared through DPPH, ABTS, and reducing power assays. The anti-inflammatory activity was evaluated by measuring the inhibitory effects on lipopolysaccharide-induced secretion of nitric oxide (NO), interleukin-6 (IL -6), and tumor necrosis factor -alpha (TNF-alpha) by RAW264.7 macrophages. Additionally, compounds strongly correlated with anti-inflammatory and antioxidant activities were identified through spectrum-effect relationship analysis. Results: A total of 50 compounds were identified across the four Gentiana species. In vitro antioxidant assays demonstrated DPPH and ABTS scavenging abilities and reducing power within the concentration range of 62.5-2000 mu g/mL. All four species inhibited the production of NO, IL -6, and TNF-alpha in RAW264.7 cells. Spectrum-effect relationship analysis revealed that gentiascabraside A, gentiatibetine, tachioside, lutonarin, and isotachioside were associated with the highest antioxidant activity; and swertiamarin, tarennoside, eleganoside C, and alpigenoside were associated with the highest anti-inflammatory activity. Conclusions: This study presents, for the first time, the chemical profiles and bioactivities of G. trichotoma, G. crassuloides, and G. squarrosa, which were comprehensively compared with those of G. veitchiorum. The findings provide novel insights to understand the traditional use and/or expand the current use of Gentiana species. Additionally, this research highlights the potential of Gentiana species as natural sources of antioxidants and anti-inflammatory agents, suggesting promising applications in tea production or medicinal contexts in the near future.
英文关键词	Gentiana veitchiorum; Gentiana trichotoma; Gentiana crassuloides; Gentiana squarrosa; UPLC-QE-Orbitrap-MS; Bioactivity
语种	英语
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:001194367300001
来源期刊	JOURNAL OF ETHNOPHARMACOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/304735
作者单位	Southwest Minzu University; Southwest Minzu University
推荐引用方式 GB/T 7714	Wang, Junjun,Liu, Ruimin,Zhang, Jianguang,et al. Comparative analysis of phytochemical profile and antioxidant and anti-inflammatory activity of four Gentiana species from the Qinghai-Tibet Plateau[J],2024,326.
APA	Wang, Junjun.,Liu, Ruimin.,Zhang, Jianguang.,Su, Hongna.,Yang, Qing.,...&Lv, Zhi.(2024).Comparative analysis of phytochemical profile and antioxidant and anti-inflammatory activity of four Gentiana species from the Qinghai-Tibet Plateau.JOURNAL OF ETHNOPHARMACOLOGY,326.
MLA	Wang, Junjun,et al."Comparative analysis of phytochemical profile and antioxidant and anti-inflammatory activity of four Gentiana species from the Qinghai-Tibet Plateau".JOURNAL OF ETHNOPHARMACOLOGY 326(2024).