气候变化领域集成服务门户(CCPortal): Development of chemometric-assisted supercritical fluid extraction of effective and natural tyrosinase inhibitor from Syzygium aqueum leaves

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DOI	10.1016/j.ijbiomac.2023.129168
	Development of chemometric-assisted supercritical fluid extraction of effective and natural tyrosinase inhibitor from Syzygium aqueum leaves
	Zarudin, Nurul Husna; Normaya, Erna; Shamsuri, Syamimi Sulfiza; Iqbal, Anwar; Piah, Mohd Bijarimi Mat; Abdullah, Zanariah; Hamzah, Ahmad Sazali; Ahmad, Mohammad Norazmi
发表日期	2024
ISSN	0141-8130
EISSN	1879-0003
起始页码	258
卷号	258
英文摘要	Tyrosinase is a key enzyme in enzymatic browning, causing quality losses in food through the oxidation process. Thus, the discovery of an effective and natural tyrosinase inhibitor via green technology is of great interest to the global food market due to food security and climate change issues. In this study, Syzygium aqueum (S. aqueum) leaves, which are known to be rich in phenolic compounds (PC), were chosen as a natural source of tyrosinase inhibitor, and the effect of the sustainable, supercritical fluid extraction (SFE) process was evaluated. Response surface methodology-assisted supercritical fluid extraction (RSM-assisted SFE) was utilized to optimize the PCs extracted from S. aqueum. The highest amount of PC was obtained at the optimum conditions (55 degrees C, 3350 psi, and 70 min). The IC50 (661.815 mu g/mL) of the optimized extract was evaluated, and its antioxidant activity (96.8 %) was determined. Gas chromatography-mass spectrometry (GC-MS) results reveal that 2 ',6 '-dihydroxy-4 '-methoxychalcone (2,6-D4MC) (82.65 %) was the major PC in S. aqueum. Chemometric analysis indicated that 2,6-D4MC has similar chemical properties to the tyrosinase inhibitor control (kaempferol). The toxicity and physiochemical properties of the novel 2,6-D4MC from S. aqueum revealed that the 2,6-D4MC is safer than kaempferol as predicted via absorption, distribution, metabolism, and excretion (ADME) evaluation. Enzyme kinetic analysis shows that the type of inhibition of the optimized extract is non-competitive inhibition with Km = 1.55 mM and Vmax = 0.017 mu M/s. High-performance liquid chromatography (HPLC) analysis shows the effectiveness of S. aqueum as a tyrosinase inhibitor. The mechanistic insight of the tyrosinase inhibition using 2,6-D4MC was successfully calculated using density functional theory (DFT) and molecular docking approaches. The findings could have a significant impact on food security development by devising a sustainable and effective tyrosinase inhibitor from waste by-products that is aligned with the United Nation's SDG 2, zero hunger.
英文关键词	Syzygium aqueum; Response surface methodology; Tyrosinase inhibitor; Chemometric; Density functional theory
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science
WOS记录号	WOS:001153701400001
来源期刊	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/304165
作者单位	International Islamic University Malaysia; International Islamic University Malaysia; Universiti Sains Malaysia; Universiti Malaysia Pahang Al-Sultan Abdullah (UMPSA); Universiti Malaya; Universiti Teknologi MARA
推荐引用方式 GB/T 7714	Zarudin, Nurul Husna,Normaya, Erna,Shamsuri, Syamimi Sulfiza,et al. Development of chemometric-assisted supercritical fluid extraction of effective and natural tyrosinase inhibitor from Syzygium aqueum leaves[J],2024,258.
APA	Zarudin, Nurul Husna.,Normaya, Erna.,Shamsuri, Syamimi Sulfiza.,Iqbal, Anwar.,Piah, Mohd Bijarimi Mat.,...&Ahmad, Mohammad Norazmi.(2024).Development of chemometric-assisted supercritical fluid extraction of effective and natural tyrosinase inhibitor from Syzygium aqueum leaves.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,258.
MLA	Zarudin, Nurul Husna,et al."Development of chemometric-assisted supercritical fluid extraction of effective and natural tyrosinase inhibitor from Syzygium aqueum leaves".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 258(2024).