气候变化领域集成服务门户(CCPortal): Mix-method toolbox for monitoring greenhouse gas production and microbiome responses to soil amendments

CCPortal

DOI	10.1016/j.mex.2024.102699
	Mix-method toolbox for monitoring greenhouse gas production and microbiome responses to soil amendments
	Lourenco, Kesia Silva; Suleiman, Afnan Khalil Ahmad; Pijl, Agata; Dimitrov, Mauricio R.; Cantarella, Heitor; Kuramae, Eiko Eurya
发表日期	2024
EISSN	2215-0161
起始页码	12
卷号	12
英文摘要	In this study, we adopt an interdisciplinary approach, integrating agronomic field experiments with soil chemistry, molecular biology techniques, and statistics to investigate the impact of organic residue amendments, such as vinasse (a by-product of sugarcane ethanol production), on soil microbiome and greenhouse gas (GHG) production. The research investigates the effects of distinct disturbances, including organic residue application alone or combined with inorganic N fertilizer on the environment. The methods assess soil microbiome dynamics (composition and function), GHG emissions, and plant productivity. Detailed steps for field experimental setup, soil sampling, soil chemical analyses, determination of bacterial and fungal community diversity, quantification of genes related to nitrification and denitrification pathways, measurement and analysis of gas fluxes (N 2 O, CH 4 , and CO 2 ), and determination of plant productivity are provided. The outcomes of the methods are detailed in our publications (Louren & ccedil;o et al., 2018a; Louren & ccedil;o et al., 2018b; Louren & ccedil;o et al., 2019; Louren & ccedil;o et al., 2020). Additionally, the statistical methods and scripts used for analyzing large datasets are outlined. The aim is to assist researchers by addressing common challenges in large-scale field experiments, offering practical recommendations to avoid common pitfalls, and proposing potential analyses, thereby encouraging collaboration among diverse research groups. center dot Interdisciplinary methods and scientific questions allow for exploring broader interconnected environmental problems. center dot The proposed method can serve as a model and protocol for evaluating the impact of soil amendments on soil microbiome, GHG emissions, and plant productivity, promoting more sustainable management practices. center dot Time -series data can offer detailed insights into specific ecosystems, particularly concerning soil microbiota (taxonomy and functions).
英文关键词	Next generation sequencing (NGS); Gas measurement; Sustainability; Climate change; Global warming; Nitrous oxide; Methane; Carbon dioxide; Fungal community; Bacterial community; Dynamics of soil microbiome; Soil disturbances; Resistance and resilience; Time-series analyses; Co-occurrence of fungal and bacteria; Quantitative real-time PCR (QPCR); Vinasse; Organic residues
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001230856000001
来源期刊	METHODSX
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/303777
作者单位	Royal Netherlands Academy of Arts & Sciences; Netherlands Institute of Ecology (NIOO-KNAW); Instituto Agronomico de Campinas (IAC); Utrecht University
推荐引用方式 GB/T 7714	Lourenco, Kesia Silva,Suleiman, Afnan Khalil Ahmad,Pijl, Agata,et al. Mix-method toolbox for monitoring greenhouse gas production and microbiome responses to soil amendments[J],2024,12.
APA	Lourenco, Kesia Silva,Suleiman, Afnan Khalil Ahmad,Pijl, Agata,Dimitrov, Mauricio R.,Cantarella, Heitor,&Kuramae, Eiko Eurya.(2024).Mix-method toolbox for monitoring greenhouse gas production and microbiome responses to soil amendments.METHODSX,12.
MLA	Lourenco, Kesia Silva,et al."Mix-method toolbox for monitoring greenhouse gas production and microbiome responses to soil amendments".METHODSX 12(2024).