气候变化领域集成服务门户(CCPortal): Using qPCR to Identify Potential Effects of Thermal Conditions during Embryogenesis on Mitochondrial DNA Copy Number in Juvenile Brown Trout Salmo trutta

CCPortal

DOI	10.3390/fishes9040142
	Using qPCR to Identify Potential Effects of Thermal Conditions during Embryogenesis on Mitochondrial DNA Copy Number in Juvenile Brown Trout Salmo trutta
	Erlandsson, Ann; Asmonaite, Giedre; Jonsson, Bror; Greenberg, Larry
发表日期	2024
EISSN	2410-3888
起始页码	9
结束页码	4
卷号	9 期号:4
英文摘要	Changes in the number, structure, and function of mitochondria during the early life stages of animals can play an important role for an organism's metabolic rate, growth, and health. Previous studies have shown that juvenile brown trout (Salmo trutta) subjected to elevated temperatures during the embryonic stage respond phenotypically with a reduced metabolic rate. The aim of this study was to explore if embryonic temperature affects the mitochondria content of young brown trout and as such explains the previously found differences in metabolic rates. Here, we optimize a quantitative PCR (qPCR) method for the mitochondria cytochrome c oxidase subunit I gene, and then use the method as a proxy for mitochondrial DNA content. We hypothesize that young trout subjected to elevated temperatures during the embryonic stage respond phenotypically with a reduced mitochondrial DNA content. To test this hypothesis, we subjected brown trout to either control ambient (4.4 +/- 1.5 degrees C) or elevated temperatures (7.1 +/- 0.6 degrees C) during embryogenesis. Subsequently, we extracted DNA from liver and white muscle tissue of juvenile brown trout from the two different incubation temperature treatments and successively optimized qPCR for mitochondrial DNA. We found that the amount of mitochondria DNA in liver tissue was 18 times higher than in white muscle tissue, but there was no significant difference in mitochondria content in liver or muscle tissue between brown trout exposed to elevated and ambient control temperatures during embryogenesis. We conclude that reduced metabolic rate is not likely associated with mitochondria DNA content. We also suggest that qPCR is a simple and cost-effective method to quantify mitochondria DNA in frozen and partly degraded tissue from different treatment groups and a useful proxy for identification of differences in mitochondria number.
英文关键词	COI gene; mitochondria; Salmo trutta; climate change; quantitative PCR
语种	英语
WOS研究方向	Fisheries ; Marine & Freshwater Biology
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:001211346600001
来源期刊	FISHES
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/303484
作者单位	Karlstad University; Norwegian Institute Nature Research
推荐引用方式 GB/T 7714	Erlandsson, Ann,Asmonaite, Giedre,Jonsson, Bror,et al. Using qPCR to Identify Potential Effects of Thermal Conditions during Embryogenesis on Mitochondrial DNA Copy Number in Juvenile Brown Trout Salmo trutta[J],2024,9(4).
APA	Erlandsson, Ann,Asmonaite, Giedre,Jonsson, Bror,&Greenberg, Larry.(2024).Using qPCR to Identify Potential Effects of Thermal Conditions during Embryogenesis on Mitochondrial DNA Copy Number in Juvenile Brown Trout Salmo trutta.FISHES,9(4).
MLA	Erlandsson, Ann,et al."Using qPCR to Identify Potential Effects of Thermal Conditions during Embryogenesis on Mitochondrial DNA Copy Number in Juvenile Brown Trout Salmo trutta".FISHES 9.4(2024).