气候变化领域集成服务门户(CCPortal): Environmental DNA analysis of airborne poaceae (grass) pollen reveals taxonomic diversity across seasons and climate zones

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DOI	10.1016/j.envres.2023.117983
	Environmental DNA analysis of airborne poaceae (grass) pollen reveals taxonomic diversity across seasons and climate zones
	Van Haeften, Shanice; Campbell, Bradley C.; Milic, Andelija; Addison-Smith, Elizabeth; Al Kouba, Jane; Huete, Alfredo; Beggs, Paul J.; Davies, Janet M.
发表日期	2024
ISSN	0013-9351
EISSN	1096-0953
起始页码	247
卷号	247
英文摘要	Background: Grasses populate most biogeographical zones, and their diversity influences allergic sensitisation to pollen. Previously, the contribution of different Poaceae subfamilies to airborne pollen has mostly been inferred from historical herbarium records. We recently applied environmental (e)DNA metabarcoding at one subtropical site revealing that successive airborne grass pollen peaks were derived from repeated flowering of Chloridoid and Panicoid grasses over a season. This study aimed to compare spatiotemporal patterns in grass pollen exposure across seasons and climate zones. Methods: Airborne pollen concentrations across two austral pollen seasons spanning 2017-2019 at subtropical (Mutdapilly and Rocklea, Queensland) and temperate (Macquarie Park and Richmond, New South Wales) sites, were determined with a routine volumetric impaction sampler and counting by light microscopy. Poaceae rbcL metabarcode sequences amplified from daily pollen samples collected once per week were assigned to subfamily and genus using a ribosomal classifier and compared with Atlas of Living Australia sighting records. Results: eDNA analysis revealed distinct dominance patterns of grass pollen at various sites: Panicoid grasses prevailed in both subtropical Mutdapilly and temperate Macquarie Park, whilst Chloridoid grasses dominated the subtropical Rocklea site. Overall, subtropical sites showed significantly higher proportion of pollen from Chloridoid grasses than temperate sites, whereas the temperate sites showed a significantly higher proportion of pollen from Pooideae grasses than subtropical sites. Timing of airborne Pooid (spring), Panicoid and Chloridoid (late spring to autumn), and Arundinoid (autumn) pollen were significantly related to number of days from midwinter. Proportions of eDNA for subfamilies correlated with distributions grass sighting records between climate zones. Conclusions: eDNA analysis enabled finer taxonomic discernment of Poaceae pollen records across seasons and climate zones with implications for understanding adaptation of grasslands to climate change, and the complexity of pollen exposure for patients with allergic respiratory diseases.
英文关键词	Aerobiology; Environmental DNA; Grass pollen; Metabarcoding; Poaceae; Pollen monitoring
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
WOS类目	Environmental Sciences ; Public, Environmental & Occupational Health
WOS记录号	WOS:001175394100001
来源期刊	ENVIRONMENTAL RESEARCH
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/303289
作者单位	Queensland University of Technology (QUT); Queensland University of Technology (QUT); University of Technology Sydney; Macquarie University; Queensland University of Technology (QUT); University of Queensland; University of Wollongong
推荐引用方式 GB/T 7714	Van Haeften, Shanice,Campbell, Bradley C.,Milic, Andelija,et al. Environmental DNA analysis of airborne poaceae (grass) pollen reveals taxonomic diversity across seasons and climate zones[J],2024,247.
APA	Van Haeften, Shanice.,Campbell, Bradley C..,Milic, Andelija.,Addison-Smith, Elizabeth.,Al Kouba, Jane.,...&Davies, Janet M..(2024).Environmental DNA analysis of airborne poaceae (grass) pollen reveals taxonomic diversity across seasons and climate zones.ENVIRONMENTAL RESEARCH,247.
MLA	Van Haeften, Shanice,et al."Environmental DNA analysis of airborne poaceae (grass) pollen reveals taxonomic diversity across seasons and climate zones".ENVIRONMENTAL RESEARCH 247(2024).