气候变化领域集成服务门户(CCPortal): Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the 'Epirus' Region of Greece upon Consecutive Drought Stress

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DOI	10.3390/plants13010027
	Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the 'Epirus' Region of Greece upon Consecutive Drought Stress
	Maniatis, Grigorios; Tani, Eleni; Katsileros, Anastasios; Avramidou, Evangelia V.; Pitsoli, Theodora; Sarri, Efi; Gerakari, Maria; Goufa, Maria; Panagoulakou, Maria; Xipolitaki, Konstantina; Klouvatos, Kimon; Megariti, Stamatia; Pappi, Polixeni; Papadakis, Ioannis E.; Bebeli, Penelope J.; Kapazoglou, Aliki
发表日期	2024
ISSN	2223-7747
起始页码	13
结束页码	1
卷号	13 期号:1
英文摘要	Within the framework of preserving and valorizing the rich grapevine germplasm of the Epirus region of Greece, indigenous grapevine (Vitis vinifera L.) cultivars were characterized and assessed for their resilience to abiotic stresses in the context of climate change. The cultivars 'Debina' and 'Dichali' displayed significant differences in their response to drought stress as judged by morpho-physiological analysis, indicating higher drought tolerance for Dichali. Hence, they were selected for further study aiming to identify genetic and epigenetic mechanisms possibly regulating drought adaptability. Specifically, self-rooted and heterografted on 'Richter 110' rootstock plants were subjected to two phases of drought with a recovery period in between. Gene expression analysis was performed for two stress-related miRNAs and their target genes: (a) miRNA159 and putative targets, VvMYB101, VvGATA-26-like, VvTOPLESS-4-like and (b) miRNA156 and putative target gene VvCONSTANS-5. Overall, grafted plants exhibited a higher drought tolerance than self-rooted plants, suggesting beneficial rootstock-scion interactions. Comparative analysis revealed differential gene expression under repetitive drought stresses between the two cultivars as well as between the self-rooted and grafted plants. 'Dichali' exhibited an up-regulation of most of the genes examined, which may be associated with increased tolerance. Nevertheless, the profound down-regulation of VvTOPLESS-4-like (a transcriptional co-repressor of transcription factors) upon drought and the concomitant up-regulation of miRNA159 highlights the importance of this 'miRNA-target' module in drought responsiveness. DNA methylation profiling using MSAP analysis revealed differential methylation patterns between the two genotypes in response to drought. Further investigations of gene expression and DNA methylation will contribute to our understanding of the epigenetic mechanisms underlying grapevine tolerance to drought stress.
英文关键词	abiotic stress; dehydration; grafting; rootstock; transcription factors; epigenetic regulation; miRNA; DNA methylation
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:001140438700001
来源期刊	PLANTS-BASEL
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/302925
作者单位	Agricultural University of Athens; Agricultural University of Athens
推荐引用方式 GB/T 7714	Maniatis, Grigorios,Tani, Eleni,Katsileros, Anastasios,et al. Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the 'Epirus' Region of Greece upon Consecutive Drought Stress[J],2024,13(1).
APA	Maniatis, Grigorios.,Tani, Eleni.,Katsileros, Anastasios.,Avramidou, Evangelia V..,Pitsoli, Theodora.,...&Kapazoglou, Aliki.(2024).Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the 'Epirus' Region of Greece upon Consecutive Drought Stress.PLANTS-BASEL,13(1).
MLA	Maniatis, Grigorios,et al."Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the 'Epirus' Region of Greece upon Consecutive Drought Stress".PLANTS-BASEL 13.1(2024).