气候变化领域集成服务门户(CCPortal): Fecal DNA metabarcoding helps characterize the Canada jay's diet and confirms its reliance on stored food for winter survival and breeding

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DOI	10.1371/journal.pone.0300583
	Fecal DNA metabarcoding helps characterize the Canada jay's diet and confirms its reliance on stored food for winter survival and breeding
	Sutton, Alex O.; Strickland, Dan; Lachapelle, Jacob; Young, Robert G.; Hanner, Robert; Brunton, Daniel F.; Skevington, Jeffrey H.; Freeman, Nikole E.; Norris, D. Ryan
发表日期	2024
ISSN	1932-6203
起始页码	19
结束页码	4
卷号	19 期号:4
英文摘要	Accurately determining the diet of wild animals can be challenging if food items are small, visible only briefly, or rendered visually unidentifiable in the digestive system. In some food caching species, an additional challenge is determining whether consumed diet items have been previously stored or are fresh. The Canada jay (Perisoreus canadensis) is a generalist resident of North American boreal and subalpine forests with anatomical and behavioural adaptations allowing it to make thousands of arboreal food caches in summer and fall that are presumably responsible for its high winter survival and late winter/early spring breeding. We used DNA fecal metabarcoding to obtain novel information on nestling diets and compiled a dataset of 662 published and unpublished direct observations or stomach contents identifications of natural foods consumed by Canada jays throughout the year. We then used detailed natural history information to make informed decisions on whether each item identified to species in the diets of winter adults and nestlings was best characterized as 'likely cached', 'likely fresh' (i.e., was available as a non-cached item when it appeared in a jay's feces or stomach), or 'either possible'. Of the 87 food items consumed by adults in the winter, 39% were classified as 'likely cached' and 6% were deemed to be 'likely fresh'. For nestlings, 29% of 125 food items identified to species were 'likely cached' and 38% were 'likely fresh'. Our results support both the indispensability of cached food for Canada jay winter survival and previous suggestions that cached food is important for late winter/early spring breeding. Our work highlights the value of combining metabarcoding, stomach contents analysis, and direct observations to determine the cached vs. non-cached origins of consumed food items and the identity of food caches, some of which could be especially vulnerable to degradation through climate change.
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001209049000017
来源期刊	PLOS ONE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/302882
作者单位	University of Guelph; Bangor University; Carleton University; Kansas State University
推荐引用方式 GB/T 7714	Sutton, Alex O.,Strickland, Dan,Lachapelle, Jacob,et al. Fecal DNA metabarcoding helps characterize the Canada jay's diet and confirms its reliance on stored food for winter survival and breeding[J],2024,19(4).
APA	Sutton, Alex O..,Strickland, Dan.,Lachapelle, Jacob.,Young, Robert G..,Hanner, Robert.,...&Norris, D. Ryan.(2024).Fecal DNA metabarcoding helps characterize the Canada jay's diet and confirms its reliance on stored food for winter survival and breeding.PLOS ONE,19(4).
MLA	Sutton, Alex O.,et al."Fecal DNA metabarcoding helps characterize the Canada jay's diet and confirms its reliance on stored food for winter survival and breeding".PLOS ONE 19.4(2024).