气候变化领域集成服务门户(CCPortal): High-throughput sequencing-based analysis of the composition and diversity of the endophyte community in roots of Stellera chamaejasme

CCPortal

DOI	10.1038/s41598-024-59055-x
	High-throughput sequencing-based analysis of the composition and diversity of the endophyte community in roots of Stellera chamaejasme
	Zhang, Jun-ze; Li, Xin-zhong; Yin, Ye-bing; Luo, Si-cen; Wang, Dong-xu; Zheng, Heng; Liu, Yi-xuan
发表日期	2024
ISSN	2045-2322
起始页码	14
结束页码	1
卷号	14 期号:1
英文摘要	Stellera chamaejasme (S. chamaejasme) is an important medicinal plant with heat-clearing, detoxifying, swelling and anti-inflammatory effects. At the same time, it is also one of the iconic plants of natural grassland degradation in northwest China, playing a key role in the invasion process. Plant endophytes live in healthy plant tissues and can synthesize substances needed for plant growth, induce disease resistance in host plants, and enhance plant resistance to environmental stress. Therefore, studying the root endophytes of S. chamaejasme is of great significance for mining beneficial microbial resources and biological prevention and control of S. chamaejasme. This study used Illumina MiSeq high-throughput sequencing technology to analyze the composition and diversity of endophytes in the roots of S. chamaejasme in different alpine grasslands (BGC, NMC and XGYZ) in Tibet. Research results show that the main phylum of endophytic fungi in the roots of S. chamaejasme in different regions is Ascomycota, and the main phyla of endophytic bacteria are Actinobacteria, Proteobacteria and Firmicutes (Bacteroidota). Overall, the endophyte diversity of the NMC samples was significantly higher than that of the other two sample sites. Principal coordinate analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA) results showed significant differences in the composition of endophytic bacterial and fungal communities among BGC, NMC and XGYZ samples. Co-occurrence network analysis of endophytes showed that there were positive correlations between fungi and some negative correlations between bacteria, and the co-occurrence network of bacteria was more complex than that of fungi. In short, this study provides a vital reference for further exploring and utilizing the endophyte resources of S. chamaejasme and an in-depth understanding of the ecological functions of S. chamaejasme endophytes.
英文关键词	S. chamaejasme; Endophytic bacteria; Endophytic fungi; Community structure; Composition differences
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001203416100030
来源期刊	SCIENTIFIC REPORTS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/302870
作者单位	Tibet University; Wuhan University of Technology
推荐引用方式 GB/T 7714	Zhang, Jun-ze,Li, Xin-zhong,Yin, Ye-bing,et al. High-throughput sequencing-based analysis of the composition and diversity of the endophyte community in roots of Stellera chamaejasme[J],2024,14(1).
APA	Zhang, Jun-ze.,Li, Xin-zhong.,Yin, Ye-bing.,Luo, Si-cen.,Wang, Dong-xu.,...&Liu, Yi-xuan.(2024).High-throughput sequencing-based analysis of the composition and diversity of the endophyte community in roots of Stellera chamaejasme.SCIENTIFIC REPORTS,14(1).
MLA	Zhang, Jun-ze,et al."High-throughput sequencing-based analysis of the composition and diversity of the endophyte community in roots of Stellera chamaejasme".SCIENTIFIC REPORTS 14.1(2024).