气候变化领域集成服务门户(CCPortal): A Forster Resonance Energy Transfer (FRET)-Based Immune Assay for the Detection of Microcystin-LR in Drinking Water

CCPortal

DOI	10.3390/s24103204
	A Forster Resonance Energy Transfer (FRET)-Based Immune Assay for the Detection of Microcystin-LR in Drinking Water
	Capo, Alessandro; Pennacchio, Angela; Montagnese, Concetta; Hadjiantonis, Antonis; Demosthenous, Panayiota; Giusti, Alessandro; Staiano, Maria; D'Auria, Sabato; Varriale, Antonio
发表日期	2024
EISSN	1424-8220
起始页码	24
结束页码	10
卷号	24 期号:10
英文摘要	Cyanobacteria bloom is the term used to describe an abnormal and rapid growth of cyanobacteria in aquatic ecosystems such as lakes, rivers, and oceans as a consequence of anthropic factors, ecosystem degradation, or climate change. Cyanobacteria belonging to the genera Microcystis, Anabaena, Planktothrix, and Nostoc produce and release toxins called microcystins (MCs) into the water. MCs can have severe effects on human and animal health following their ingestion and inhalation. The MC structure is composed of a constant region (composed of five amino acid residues) and a variable region (composed of two amino acid residues). When the MC variable region is composed of arginine and leucine, it is named MC-LR. The most-common methods used to detect the presence of MC-LR in water are chromatographic-based methods (HPLC, LC/MS, GC/MS) and immunological-based methods (ELISA). In this work, we developed a new competitive F & ouml;rster resonance energy transfer (FRET) assay to detect the presence of traces of MC-LR in water. Monoclonal antibody anti-MC-LR and MC-LR conjugated with bovine serum albumin (BSA) were labeled with the near-infrared fluorophores CF568 and CF647, respectively. Steady-state fluorescence measurements were performed to investigate the energy transfer process between anti-MC-LR 568 and MC-LR BSA 647 upon their interaction. Since the presence of unlabeled MC-LR competes with the labeled one, a lower efficiency of FRET process can be observed in the presence of an increasing amount of unlabeled MC-LR. The limit of detection (LoD) of the FRET assay is found to be 0.245 nM (0.245 mu g/L). This value is lower than the provisional limit established by the World Health Organization (WHO) for quantifying the presence of MC-LR in drinking water.
英文关键词	biosensors; Cyanobacteria bloom; microcystin; FRET; fluorescence spectroscopy
语种	英语
WOS研究方向	Chemistry ; Engineering ; Instruments & Instrumentation
WOS类目	Chemistry, Analytical ; Engineering, Electrical & Electronic ; Instruments & Instrumentation
WOS记录号	WOS:001231423300001
来源期刊	SENSORS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/302705
作者单位	Consiglio Nazionale delle Ricerche (CNR); Istituto di Scienze dell' Alimentazione (ISA-CNR); Consiglio Nazionale delle Ricerche (CNR); Istituto di Scienze dell' Alimentazione (ISA-CNR); Consiglio Nazionale delle Ricerche (CNR)
推荐引用方式 GB/T 7714	Capo, Alessandro,Pennacchio, Angela,Montagnese, Concetta,et al. A Forster Resonance Energy Transfer (FRET)-Based Immune Assay for the Detection of Microcystin-LR in Drinking Water[J],2024,24(10).
APA	Capo, Alessandro.,Pennacchio, Angela.,Montagnese, Concetta.,Hadjiantonis, Antonis.,Demosthenous, Panayiota.,...&Varriale, Antonio.(2024).A Forster Resonance Energy Transfer (FRET)-Based Immune Assay for the Detection of Microcystin-LR in Drinking Water.SENSORS,24(10).
MLA	Capo, Alessandro,et al."A Forster Resonance Energy Transfer (FRET)-Based Immune Assay for the Detection of Microcystin-LR in Drinking Water".SENSORS 24.10(2024).