气候变化领域集成服务门户(CCPortal): Simultaneous species detection and discovery with environmental DNA metabarcoding: A freshwater mollusk case study

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DOI	10.1002/ece3.11020
	Simultaneous species detection and discovery with environmental DNA metabarcoding: A freshwater mollusk case study
	Vanderpool, Daniel D.; Wilcox, Taylor M.; Young, Michael K.; Pilgrim, Kristine L.; Schwartz, Michael K.
发表日期	2024
ISSN	2045-7758
起始页码	14
结束页码	2
卷号	14 期号:2
英文摘要	Environmental DNA (eDNA) sampling is a powerful tool for rapidly characterizing biodiversity patterns for specious, cryptic taxa with incomplete taxonomies. One such group that are also of high conservation concern are North American freshwater gastropods. In particular, springsnails of the genus Pyrgulopsis (Family: Hydrobiidae) are prevalent throughout the western United States where >140 species have been described. Many of the described species are narrow endemics known from a single spring or locality, and it is believed that there are likely many additional species which have yet to be described. The distribution of these species across the landscape is of interest because habitat loss and degradation, climate change, groundwater mining, and pollution have resulted in springsnail imperilment rates as high as 92%. Determining distributions with conventional sampling methods is limited by the fact that these snails are often <5 mm in length with few distinguishing morphological characters, making them both difficult to detect and to identify. We developed an eDNA metabarcoding protocol that is both inexpensive and capable of rapid, accurate detection of all known Pyrgulopsis species. When compared with conventional collection techniques, our pipeline consistently resulted in detection at sites previously known to contain Pyrgulopsis springsnails and at a cost per site that is likely to be substantially less than the conventional sampling and individual barcoding that has been done historically. Additionally, because our method uses eDNA extracted from filtered water, it is non-destructive and suitable for the detection of endangered species where no take restrictions may be in effect. This effort represents both a tool which is immediately applicable to taxa of high conservation concern across western North America and a case study in the broader application of eDNA sampling for landscape assessments of cryptic taxa of conservation concern.
英文关键词	environmental DNA species detection; metabarcoding; springsnails
语种	英语
WOS研究方向	Environmental Sciences & Ecology ; Evolutionary Biology
WOS类目	Ecology ; Evolutionary Biology
WOS记录号	WOS:001163395500001
来源期刊	ECOLOGY AND EVOLUTION
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/302509
作者单位	United States Department of Agriculture (USDA); United States Forest Service
推荐引用方式 GB/T 7714	Vanderpool, Daniel D.,Wilcox, Taylor M.,Young, Michael K.,et al. Simultaneous species detection and discovery with environmental DNA metabarcoding: A freshwater mollusk case study[J],2024,14(2).
APA	Vanderpool, Daniel D.,Wilcox, Taylor M.,Young, Michael K.,Pilgrim, Kristine L.,&Schwartz, Michael K..(2024).Simultaneous species detection and discovery with environmental DNA metabarcoding: A freshwater mollusk case study.ECOLOGY AND EVOLUTION,14(2).
MLA	Vanderpool, Daniel D.,et al."Simultaneous species detection and discovery with environmental DNA metabarcoding: A freshwater mollusk case study".ECOLOGY AND EVOLUTION 14.2(2024).