气候变化领域集成服务门户(CCPortal): Biochemical and proteomic response of the freshwater green alga Pseudochlorella pringsheimii to iron and salinity stressors

CCPortal

DOI	10.1186/s12870-023-04688-9
	Biochemical and proteomic response of the freshwater green alga Pseudochlorella pringsheimii to iron and salinity stressors
	Ismaiel, Mostafa M. S.; Piercey-Normore, Michele D.; Rampitsch, Christof
发表日期	2024
ISSN	1471-2229
起始页码	24
结束页码	1
卷号	24 期号:1
英文摘要	BackgroundPseudochlorella pringsheimii (Ppr) is a green unicellular alga rich with chlorophyll, carotenoids, and antioxidants. As a widespread organism, Ppr must face, and adapt to, many environmental stresses and these are becoming more frequent and more extreme under the conditions of climate change. We therefore focused on salinity induced by NaCl and iron (Fe) variation stresses, which are commonly encountered by algae in their natural environment.ResultsThe relatively low stress levels improved the biomass, growth rate, and biochemical components of Ppr. In addition, the radical-scavenging activity, reducing power, and chelating activity were stimulated by lower iron concentrations and all NaCl concentrations. We believe that the alga has adapted to the stressors by increasing certain biomolecules such as carotenoids, phenolics, proteins, and carbohydrates. These act as antioxidants and osmoregulators to protect cell membranes and other cellular components from the harmful effects of ions. We have used SDS-PAGE and 2D-PAGE in combination with tandem mass spectrometry to identify responsive proteins in the proteomes of stressed vs. non-stressed Ppr. The results of 2D-PAGE analysis showed a total of 67 differentially expressed proteins, and SDS-PAGE identified 559 peptides corresponding to 77 proteins. Of these, 15, 8, and 17 peptides were uniquely identified only under the control, iron, and salinity treatments, respectively. The peptides were classified into 12 functional categories: energy metabolism (the most notable proteins), carbohydrate metabolism, regulation, photosynthesis, protein synthesis, stress proteins, oxido-reductase proteins, transfer proteins, ribonucleic-associated proteins, hypothetical proteins, and unknown proteins. The number of identified peptides was higher under salinity stress compared to iron stress.ConclusionsA proposed mechanism for the adaptation of Ppr to stress is discussed based on the collected data. This data could serve as reference material for algal proteomics and the mechanisms involved in mediating stress tolerance.
英文关键词	Antioxidants; ATP; Carbohydrate; Metabolism; Microalgae; Stress
语种	英语
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:001139133700002
来源期刊	BMC PLANT BIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/302386
作者单位	Egyptian Knowledge Bank (EKB); Zagazig University; Algoma University; Agriculture & Agri Food Canada
推荐引用方式 GB/T 7714	Ismaiel, Mostafa M. S.,Piercey-Normore, Michele D.,Rampitsch, Christof. Biochemical and proteomic response of the freshwater green alga Pseudochlorella pringsheimii to iron and salinity stressors[J],2024,24(1).
APA	Ismaiel, Mostafa M. S.,Piercey-Normore, Michele D.,&Rampitsch, Christof.(2024).Biochemical and proteomic response of the freshwater green alga Pseudochlorella pringsheimii to iron and salinity stressors.BMC PLANT BIOLOGY,24(1).
MLA	Ismaiel, Mostafa M. S.,et al."Biochemical and proteomic response of the freshwater green alga Pseudochlorella pringsheimii to iron and salinity stressors".BMC PLANT BIOLOGY 24.1(2024).