气候变化领域集成服务门户(CCPortal): Development of a Thermodynamically Favorable Multi-enzyme Cascade Reaction for Efficient Sustainable Production of ω-Amino Fatty Acids and α,ω-Diamines

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DOI	10.1002/cssc.202301477
	Development of a Thermodynamically Favorable Multi-enzyme Cascade Reaction for Efficient Sustainable Production of ω-Amino Fatty Acids and α,ω-Diamines
	Li, Ju-Mou; Shi, Kun; Li, Ai-Tao; Zhang, Zhi-Jun; Yu, Hui-Lei; Xu, Jian-He
发表日期	2024
ISSN	1864-5631
EISSN	1864-564X
起始页码	17
结束页码	6
卷号	17 期号:6
英文摘要	Aliphatic omega-amino fatty acids (omega-AFAs) and alpha,omega-diamines (alpha,omega-DMs) are essential monomers for the production of nylons. Development of a sustainable biosynthesis route for omega-AFAs and alpha,omega-DMs is crucial in addressing the challenges posed by climate change. Herein, we constructed an unprecedented thermodynamically favorable multi-enzyme cascade (TherFavMEC) for the efficient sustainable biosynthesis of omega-AFAs and alpha,omega-DMs from cheap alpha,omega-dicarboxylic acids (alpha,omega-DAs). This TherFavMEC was developed by incorporating bioretrosynthesis analysis tools, reaction Gibbs free energy calculations, thermodynamic equilibrium shift strategies and cofactor (NADPH&ATP) regeneration systems. The molar yield of 6-aminohexanoic acid (6-ACA) from adipic acid (AA) was 92.3 %, while the molar yield from 6-ACA to 1,6-hexanediamine (1,6-HMD) was 96.1 %, which were significantly higher than those of previously reported routes. Furthermore, the biosynthesis of omega-AFAs and alpha,omega-DMs from 20.0 mM alpha,omega-DAs (C6-C9) was also performed, giving 11.2 mM 1,6-HMD (56.0 % yield), 14.8 mM 1,7-heptanediamine (74.0 % yield), 17.4 mM 1,8-octanediamine (87.0 % yield), and 19.7 mM 1,9-nonanediamine (98.5 % yield), respectively. The titers of 1,9-nonanediamine, 1,8-octanediamine, 1,7-heptanediamine and 1,6-HMD were improved by 328-fold, 1740-fold, 87-fold and 3.8-fold compared to previous work. Therefore, this work holds great potential for the bioproduction of omega-AFAs and alpha,omega-DMs.
英文关键词	multi-enzyme cascade; carboxylic acid reductase; omega-transaminase; cofactor regeneration; nylon monomer
语种	英语
WOS研究方向	Chemistry ; Science & Technology - Other Topics
WOS类目	Chemistry, Multidisciplinary ; Green & Sustainable Science & Technology
WOS记录号	WOS:001144017600001
来源期刊	CHEMSUSCHEM
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/301650
作者单位	East China University of Science & Technology; Hubei University
推荐引用方式 GB/T 7714	Li, Ju-Mou,Shi, Kun,Li, Ai-Tao,et al. Development of a Thermodynamically Favorable Multi-enzyme Cascade Reaction for Efficient Sustainable Production of ω-Amino Fatty Acids and α,ω-Diamines[J],2024,17(6).
APA	Li, Ju-Mou,Shi, Kun,Li, Ai-Tao,Zhang, Zhi-Jun,Yu, Hui-Lei,&Xu, Jian-He.(2024).Development of a Thermodynamically Favorable Multi-enzyme Cascade Reaction for Efficient Sustainable Production of ω-Amino Fatty Acids and α,ω-Diamines.CHEMSUSCHEM,17(6).
MLA	Li, Ju-Mou,et al."Development of a Thermodynamically Favorable Multi-enzyme Cascade Reaction for Efficient Sustainable Production of ω-Amino Fatty Acids and α,ω-Diamines".CHEMSUSCHEM 17.6(2024).