气候变化领域集成服务门户(CCPortal): Genome-Wide Identification and Transcriptional Analysis of AP2/ERF Gene Family in Pearl Millet (Pennisetum glaucum)

CCPortal

DOI	10.3390/ijms25052470
	Genome-Wide Identification and Transcriptional Analysis of AP2/ERF Gene Family in Pearl Millet (Pennisetum glaucum)
	Xu, Liang; Lan, Ying; Lin, Miaohong; Zhou, Hongkai; Ying, Sheng; Chen, Miao
发表日期	2024
ISSN	1661-6596
EISSN	1422-0067
起始页码	25
结束页码	5
卷号	25 期号:5
英文摘要	The apetala2/ethylene response factor (AP2/ERF) gene family plays a crucial role in regulating plant growth and development and responding to different abiotic stresses (e.g., drought, heat, cold, and salinity). However, the knowledge of the ERF family in pearl millet remains limited. Here, a total of 167 high-confidence PgERF genes are identified and divided into five subgroups based on gene-conserved structure and phylogenetic analysis. Forty-one pairs of segmental duplication are found using collinear analysis. Nucleotide substitution analysis reveals these duplicated pairs are under positive purification, indicating they are actively responding to natural selection. Comprehensive transcriptomic analysis reveals that PgERF genesare preferentially expressed in the imbibed seeds and stem (tilling stage) and respond to heat, drought, and salt stress. Prediction of the cis-regulatory element by the PlantCARE program indicates that PgERF genes are involved in responses to environmental stimuli. Using reverse transcription quantitative real-time PCR (RT-qPCR), expression profiles of eleven selected PgERF genes are monitored in various tissues and during different abiotic stresses. Transcript levels of each PgERF gene exhibit significant changes during stress treatments. Notably, the PgERF7 gene is the only candidate that can be induced by all adverse conditions. Furthermore, four PgERF genes (i.e., PgERF22, PgERF37, PgERF88, and PgERF155) are shown to be involved in the ABA-dependent signaling pathway. These results provide useful bioinformatic and transcriptional information for understanding the roles of the pearl millet ERF gene family in adaptation to climate change.
英文关键词	abiotic stresses; dehydration-responsive element binding protein (DREB); ethylene response factor (ERF); pearl millet; transcriptionalanalysis
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:001183281200001
来源期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/301458
作者单位	Guangdong Ocean University; Michigan State University; Guangdong Ocean University
推荐引用方式 GB/T 7714	Xu, Liang,Lan, Ying,Lin, Miaohong,et al. Genome-Wide Identification and Transcriptional Analysis of AP2/ERF Gene Family in Pearl Millet (Pennisetum glaucum)[J],2024,25(5).
APA	Xu, Liang,Lan, Ying,Lin, Miaohong,Zhou, Hongkai,Ying, Sheng,&Chen, Miao.(2024).Genome-Wide Identification and Transcriptional Analysis of AP2/ERF Gene Family in Pearl Millet (Pennisetum glaucum).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(5).
MLA	Xu, Liang,et al."Genome-Wide Identification and Transcriptional Analysis of AP2/ERF Gene Family in Pearl Millet (Pennisetum glaucum)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.5(2024).