气候变化领域集成服务门户(CCPortal): Functional transcriptome analysis revealed upregulation of MAPK-SMAD signalling pathways in chronic heat stress in crossbred cattle

CCPortal

DOI	10.1007/s00484-024-02672-y
	Functional transcriptome analysis revealed upregulation of MAPK-SMAD signalling pathways in chronic heat stress in crossbred cattle
	Dutta, Gaurav; Alex, Rani; Singh, Ayushi; Gowane, Gopal R.; Vohra, Vikas; De, Sachidanandan; Verma, Archana; Ludri, Ashutosh
发表日期	2024
ISSN	0020-7128
EISSN	1432-1254
英文摘要	Animal geneticists and breeders have the impending challenge of enhancing the resilience of Indian livestock to heat stress through better selection strategies. Climate change's impact on livestock is more intense in tropical countries like India where dairy cattle crossbreeds are more sensitive to heat stress. The main reason for this study was to find the missing relative changes in transcript levels in thermo-neutral and heat stress conditions in crossbred cattle through whole-transcriptome analysis of RNA-Seq data. Differentially expressed genes (DEGs) identified based on the minimum log twofold change value and false discovery rate 0.05 revealed 468 up-regulated genes and 2273 down-regulated significant genes. Functional annotation and pathway analysis of these significant DEGs were compared based on Gene Ontology (Biological process), Kyoto Encyclopedia of Genes and Genome (KEGG), and Reactome pathways using g: Profiler, ShinyGO v0.76, and iDEP.951 web tools. On finding network visualization, the most over-represented and correlated pathways were neuronal and sensory organ development, calcium signalling pathway, Mitogen-activated protein kinase (MAPK) and Smad signalling pathway, Ras-proximate-1, or Ras-related protein 1 (Rap 1) signalling pathway, apoptosis, and oxidative stress. Similarly, down-regulated genes were most expressed in mRNA processing, immune system, B-cell receptor signalling pathway, Nucleotide oligomerization domain (NOD)-like receptors (NLRs) signalling pathway and nonsense-mediated decay (NMD) pathway. The heat stress-responsive genes identified in this study will facilitate our understanding of the molecular basis for climate resilience and heat tolerance in Indian dairy crossbreeds.
英文关键词	Transcriptome; Karan Fries; mRNA processing; Oxidative stress; MAPK and Smad signalling pathway
语种	英语
WOS研究方向	Biophysics ; Environmental Sciences & Ecology ; Meteorology & Atmospheric Sciences ; Physiology
WOS类目	Biophysics ; Environmental Sciences ; Meteorology & Atmospheric Sciences ; Physiology
WOS记录号	WOS:001216016200001
来源期刊	INTERNATIONAL JOURNAL OF BIOMETEOROLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/301419
作者单位	Indian Council of Agricultural Research (ICAR); ICAR - National Dairy Research Institute
推荐引用方式 GB/T 7714	Dutta, Gaurav,Alex, Rani,Singh, Ayushi,et al. Functional transcriptome analysis revealed upregulation of MAPK-SMAD signalling pathways in chronic heat stress in crossbred cattle[J],2024.
APA	Dutta, Gaurav.,Alex, Rani.,Singh, Ayushi.,Gowane, Gopal R..,Vohra, Vikas.,...&Ludri, Ashutosh.(2024).Functional transcriptome analysis revealed upregulation of MAPK-SMAD signalling pathways in chronic heat stress in crossbred cattle.INTERNATIONAL JOURNAL OF BIOMETEOROLOGY.
MLA	Dutta, Gaurav,et al."Functional transcriptome analysis revealed upregulation of MAPK-SMAD signalling pathways in chronic heat stress in crossbred cattle".INTERNATIONAL JOURNAL OF BIOMETEOROLOGY (2024).