气候变化领域集成服务门户(CCPortal): Comparison of two different experimental environments for resistance screenings for the leafhopper-transmitted wheat dwarf virus in wheat

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DOI	10.1007/s41348-024-00929-7
	Comparison of two different experimental environments for resistance screenings for the leafhopper-transmitted wheat dwarf virus in wheat
	Pfrieme, Anne-Kathrin; Stahl, Andreas; Pillen, Klaus; Will, Torsten
发表日期	2024
ISSN	1861-3829
EISSN	1861-3837
英文摘要	Wheat dwarf virus (WDV) causes high yield losses in wheat and other cereals and is therefore an important pathogen transmitted by the leafhopper Psammotettix alienus. Climate change will increase infections by insect-transmitted viruses due to the increasing spread of vectors. In the context of integrated pest management, the cultivation of WDV-resistant/tolerant varieties is an effective way of controlling WDV. Evaluation of tolerant/resistant genotypes is based on inoculation with viruliferous leafhoppers and subsequent phenotyping in gauze houses under semi-field conditions. For successful screening, it is important to ensure the uniform and reproducible inoculation of plants. Abiotic conditions, particularly temperature, have a critical influence on inoculation success, and thus, variations in infection rates were observed within and between previous replicates in the field. Furthermore, the leafhopper population reared in the greenhouse has to be reestablished after each infection, which delays the screening process. We addressed these issues by developing an improved inoculation assay in which plants are inoculated in small infection hoods in the greenhouse before being planted out in gauze houses. This procedure allows optimal environmental conditions for WDV infection of test plants and allows the plants with WDV infection to develop under natural environmental conditions for symptom scoring. In addition, the viruliferous leafhoppers were recollected from the test plants after infection, allowing a sustainable use of the insects. The method thus enables more reliable phenotyping by increasing infection success and testing a greater number of genotypes in a shorter time.
英文关键词	Resistance breeding; Screening for resistance; Phenotyping; Plant virus
语种	英语
WOS研究方向	Agriculture ; Plant Sciences
WOS类目	Agriculture, Multidisciplinary ; Plant Sciences
WOS记录号	WOS:001235034800001
来源期刊	JOURNAL OF PLANT DISEASES AND PROTECTION
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/301413
作者单位	Julius Kuhn-Institut; Martin Luther University Halle Wittenberg
推荐引用方式 GB/T 7714	Pfrieme, Anne-Kathrin,Stahl, Andreas,Pillen, Klaus,et al. Comparison of two different experimental environments for resistance screenings for the leafhopper-transmitted wheat dwarf virus in wheat[J],2024.
APA	Pfrieme, Anne-Kathrin,Stahl, Andreas,Pillen, Klaus,&Will, Torsten.(2024).Comparison of two different experimental environments for resistance screenings for the leafhopper-transmitted wheat dwarf virus in wheat.JOURNAL OF PLANT DISEASES AND PROTECTION.
MLA	Pfrieme, Anne-Kathrin,et al."Comparison of two different experimental environments for resistance screenings for the leafhopper-transmitted wheat dwarf virus in wheat".JOURNAL OF PLANT DISEASES AND PROTECTION (2024).