气候变化领域集成服务门户(CCPortal): Evolution of a Restriction Factor by Domestication of a Yeast Retrotransposon

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DOI	10.1093/molbev/msae050
	Evolution of a Restriction Factor by Domestication of a Yeast Retrotransposon
	Hannon-Hatfield, J. Adam; Chen, Jingxuan; Bergman, Casey M.; Garfinkel, David J.
发表日期	2024
ISSN	0737-4038
EISSN	1537-1719
起始页码	41
结束页码	3
卷号	41 期号:3
英文摘要	Transposable elements drive genome evolution in all branches of life. Transposable element insertions are often deleterious to their hosts and necessitate evolution of control mechanisms to limit their spread. The long terminal repeat retrotransposon Ty1 prime (Ty1 '), a subfamily of the Ty1 family, is present in many Saccharomyces cerevisiae strains, but little is known about what controls its copy number. Here, we provide evidence that a novel gene from an exapted Ty1 ' sequence, domesticated restriction of Ty1 ' relic 2 (DRT2), encodes a restriction factor that inhibits Ty1 ' movement. DRT2 arose through domestication of a Ty1 ' GAG gene and contains the C-terminal domain of capsid, which in the related Ty1 canonical subfamily functions as a self-encoded restriction factor. Bioinformatic analysis reveals the widespread nature of DRT2, its evolutionary history, and pronounced structural variation at the Ty1 ' relic 2 locus. Ty1 ' retromobility analyses demonstrate DRT2 restriction factor functionality, and northern blot and RNA-seq analysis indicate that DRT2 is transcribed in multiple strains. Velocity cosedimentation profiles indicate an association between Drt2 and Ty1 ' virus-like particles or assembly complexes. Chimeric Ty1 ' elements containing DRT2 retain retromobility, suggesting an ancestral role of productive Gag C-terminal domain of capsid functionality is present in the sequence. Unlike Ty1 canonical, Ty1 ' retromobility increases with copy number, suggesting that C-terminal domain of capsid-based restriction is not limited to the Ty1 canonical subfamily self-encoded restriction factor and drove the endogenization of DRT2. The discovery of an exapted Ty1 ' restriction factor provides insight into the evolution of the Ty1 family, evolutionary hot-spots, and host-transposable element interactions.
英文关键词	retrotransposon; host defense; Saccharomyces; domesticated gene
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Evolutionary Biology ; Genetics & Heredity
WOS记录号	WOS:001191312300017
来源期刊	MOLECULAR BIOLOGY AND EVOLUTION
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/300585
作者单位	University System of Georgia; University of Georgia; University System of Georgia; University of Georgia; University System of Georgia; University of Georgia
推荐引用方式 GB/T 7714	Hannon-Hatfield, J. Adam,Chen, Jingxuan,Bergman, Casey M.,et al. Evolution of a Restriction Factor by Domestication of a Yeast Retrotransposon[J],2024,41(3).
APA	Hannon-Hatfield, J. Adam,Chen, Jingxuan,Bergman, Casey M.,&Garfinkel, David J..(2024).Evolution of a Restriction Factor by Domestication of a Yeast Retrotransposon.MOLECULAR BIOLOGY AND EVOLUTION,41(3).
MLA	Hannon-Hatfield, J. Adam,et al."Evolution of a Restriction Factor by Domestication of a Yeast Retrotransposon".MOLECULAR BIOLOGY AND EVOLUTION 41.3(2024).