气候变化领域集成服务门户(CCPortal): Cross-species gut microbiota transplantation predictably affects host heat tolerance

CCPortal

DOI	10.1242/jeb.246735
	Cross-species gut microbiota transplantation predictably affects host heat tolerance
	Dallas, Jason W.; Kazarina, Anna; Lee, Sonny T. M.; Warne, Robin W.
发表日期	2024
ISSN	0022-0949
EISSN	1477-9145
起始页码	227
结束页码	1
卷号	227 期号:1
英文摘要	The gut microbiome is known to influence and have regulatory effects in diverse physiological functions of host animals, but only recently has the relationship between host thermal biology and gut microbiota been explored. Here, we examined how early-life manipulations of the gut microbiota in larval amphibians influenced their critical thermal maximum (CTmax) at different acclimation temperatures. We stripped the resident microbiome from egg masses of wild-caught wood frogs (Lithobates sylvaticus) via an antibiotic wash, and then inoculated the eggs with pond water (control), no inoculation, or the intestinal microbiota of another species that has a wider thermal tolerance - green frogs (Lithobates clamitans). We predicted that this crossspecies transplant would increase the CTmax of the recipient wood frog larvae relative to the other treatments. In line with this prediction, green frog microbiome-recipient larvae had the highest CTmax while those with no inoculum had the lowest CTmax. Both the microbiome treatment and acclimation temperature significantly influenced the larval gut microbiota communities and alpha-diversity indices. Green frog microbiome-inoculated larvae were enriched in Rikenellaceae relative to the other treatments, which produce short-chain fatty acids and could contribute to greater energy availability and enhanced heat tolerance. Larvae that received no inoculation had a higher relative abundance of potentially pathogenic Aeromonas spp., which negatively affects host health and performance. Our results are the first to show that cross-species gut microbiota transplants alter heat tolerance in a predictable manner. This finding has repercussions for the conservation of species that are threatened by climate change and demonstrates a need to further explore the mechanisms by which the gut microbiota modulate host thermal tolerance.
英文关键词	CTmax; Thermal acclimation; Gut microbiota; Wood frogs; Microbiome manipulation
语种	英语
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Zoology
WOS类目	Biology ; Zoology
WOS记录号	WOS:001214515700003
来源期刊	JOURNAL OF EXPERIMENTAL BIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/300221
作者单位	Southern Illinois University System; Southern Illinois University; Kansas State University; Middle Tennessee State University
推荐引用方式 GB/T 7714	Dallas, Jason W.,Kazarina, Anna,Lee, Sonny T. M.,et al. Cross-species gut microbiota transplantation predictably affects host heat tolerance[J],2024,227(1).
APA	Dallas, Jason W.,Kazarina, Anna,Lee, Sonny T. M.,&Warne, Robin W..(2024).Cross-species gut microbiota transplantation predictably affects host heat tolerance.JOURNAL OF EXPERIMENTAL BIOLOGY,227(1).
MLA	Dallas, Jason W.,et al."Cross-species gut microbiota transplantation predictably affects host heat tolerance".JOURNAL OF EXPERIMENTAL BIOLOGY 227.1(2024).