气候变化领域集成服务门户(CCPortal): Effects of chronic cold stress on tissue structure, antioxidant response, and key gene expression in the warm-water bivalve Chlamys nobilis

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DOI	10.1016/j.cbd.2024.101225
	Effects of chronic cold stress on tissue structure, antioxidant response, and key gene expression in the warm-water bivalve Chlamys nobilis
	Zhang, Chuanxu; Sun, Yizhou; Wen, Jiahua; Xu, Boya; Zhu, Wenlu; Zhang, Hongkuan; Liu, Xiaodong; LiChu, Lingshan; Zheng, Huaiping
发表日期	2024
ISSN	1744-117X
EISSN	1878-0407
起始页码	50
卷号	50
英文摘要	As ectothermic invertebrates, mollusks are regarded as good environmental indicator species for determining the adverse effects of climate change on marine organisms. In the present study, the effects of cold stress on the tissue structure, antioxidant activity, and expression levels of genes were evaluated in the warm-water noble scallop Chlamys nobilis by simulating natural seawater cooled down during winter from 17 degrees C to 14 degrees C, 12 degrees C, 10 degrees C, and 9 degrees C. Firstly, the gill was severely damaged at 10 degrees C and 9 degrees C, indicating that it could be used as a visually indicative organ for monitoring cold stress. The methylenedioxyamphetamine (MDA) content significantly increased with the temperatures decreasing, meanwhile, the antioxidant enzyme activities superoxide dismutase (SOD) and catalase (CAT) showed a similar pattern, suggesting that the scallop made a positive response. More importantly, 6179 genes related to low temperatures were constructed in a module-gene clustering heat map including 10 modules. Furthermore, three gene modules about membrane lipid metabolism, amino acid metabolism, and molecular defense were identified. Finally, six key genes were verified, and HEATR1, HSP70B2, PI3K, and ATP6V1B were significantly upregulated, while WNT6 and SHMT were significantly downregulated under cold stress. This study provides a dynamic demonstration of the major gene pathways' response to various lowtemperature stresses from a transcriptomic perspective. The findings shed light on how warm-water bivalves can tolerate cold stress and can help in breeding new strains of aquatic organisms with low-temperature resistance.
英文关键词	Cold stress; Warm-water Chlamys nobilis; Gill; Antioxidant response; Gene
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:001207094700001
来源期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/299768
作者单位	Shantou University; Shantou University; Shantou University
推荐引用方式 GB/T 7714	Zhang, Chuanxu,Sun, Yizhou,Wen, Jiahua,et al. Effects of chronic cold stress on tissue structure, antioxidant response, and key gene expression in the warm-water bivalve Chlamys nobilis[J],2024,50.
APA	Zhang, Chuanxu.,Sun, Yizhou.,Wen, Jiahua.,Xu, Boya.,Zhu, Wenlu.,...&Zheng, Huaiping.(2024).Effects of chronic cold stress on tissue structure, antioxidant response, and key gene expression in the warm-water bivalve Chlamys nobilis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,50.
MLA	Zhang, Chuanxu,et al."Effects of chronic cold stress on tissue structure, antioxidant response, and key gene expression in the warm-water bivalve Chlamys nobilis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 50(2024).