气候变化领域集成服务门户(CCPortal): MiR408 balances and heat in rice

CCPortal

DOI	10.1016/j.envexpbot.2024.105717
	MiR408 balances and heat in rice
	Wen, Fengting; Gao, Yu; Zeng, Yuxiang; Li, Guangyan; Feng, Baohua; Li, Hubo; Chen, Tingting; Wang, Danying; Tao, Longxing; Xiong, Jie; Fu, Guanfu
发表日期	2024
ISSN	0098-8472
EISSN	1873-7307
起始页码	221
卷号	221
英文摘要	MicroRNAs are small noncoding RNAs that regulate gene expression by targeting complementary mRNA molecules. Among them, microRNA408 (miR408) is an ancient microRNA influencing plant growth and aiding in abiotic stress adaptation. We proved that miR408 sliced uclacyanin7 ( UCL7 ) directly by degradome sequencing. However, the mechanisms underlying the balance between growth and stress response of rice ( Oryza sativa L.) controlled by miR408 remain unclear. We conducted a study comparing Nipponbare (wild -type, WT) plants with miR408 - 3p - overexpression (miR408 - 3p-OX) and UCL7 -overexpression (UCL7 - OX) plants under normal and heat stress conditions. Through RNA -sequencing, physiological measurements, and phenotype examination, we observed that miR408 overexpression resulted in increased dry weight and grain yield, higher levels of ATP and non-structural carbohydrates (NSC), improved photosynthesis, and reduced H 2 O 2 content, antioxidant enzyme activity, and ATPase activity in seedling plants compared to UCL7-OX plants. Additionally, the application of H 2 O 2 scavengers, such as ascorbic acid (ASA) and dimethylthiourea (DMTU), further enhanced dry weight and photosynthesis by reducing H 2 O 2 levels. Under heat stress conditions, miR408 overexpression sharply increased H 2 O 2 content, antioxidant enzyme activity, ATP levels, and ATPase activity compared to UCL7-OX plants, thereby enhancing heat tolerance in rice seedlings. These findings suggest that miR408 and its target gene UCL7 participate in regulating energy homeostasis to achieve a balance between growth and heat response. This regulation involves modulating H 2 O 2 levels and optimizing the relationship between the target of rapamycin (TOR) and SNF1-related protein kinase 1 (SnRK1) in rice.
英文关键词	Energy homeostasis; MiR408; Plant growth; Rice(Oryza sativa L.); Heat response
语种	英语
WOS研究方向	Plant Sciences ; Environmental Sciences & Ecology
WOS类目	Plant Sciences ; Environmental Sciences
WOS记录号	WOS:001220276800001
来源期刊	ENVIRONMENTAL AND EXPERIMENTAL BOTANY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/299667
作者单位	Zhejiang Sci-Tech University; Chinese Academy of Agricultural Sciences; China National Rice Research Institute, CAAS; Chinese Academy of Agricultural Sciences; Institute of Cotton Research, CAAS; Yangzhou University
推荐引用方式 GB/T 7714	Wen, Fengting,Gao, Yu,Zeng, Yuxiang,et al. MiR408 balances and heat in rice[J],2024,221.
APA	Wen, Fengting.,Gao, Yu.,Zeng, Yuxiang.,Li, Guangyan.,Feng, Baohua.,...&Fu, Guanfu.(2024).MiR408 balances and heat in rice.ENVIRONMENTAL AND EXPERIMENTAL BOTANY,221.
MLA	Wen, Fengting,et al."MiR408 balances and heat in rice".ENVIRONMENTAL AND EXPERIMENTAL BOTANY 221(2024).