气候变化领域集成服务门户(CCPortal): Flexible B12 ecophysiology of Phaeocystis antarctica due to a fusion B12-independent methionine synthase with widespread homologues

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DOI	10.1073/pnas.2204075121
	Flexible B12 ecophysiology of Phaeocystis antarctica due to a fusion B12-independent methionine synthase with widespread homologues
	Rao, Deepa; Fussy, Zoltan; Brisbin, Margaret M.; McIlvin, Matthew R.; Moran, Dawn M.; Allen, Andrew E.; Follows, Michael J.; Saito, MakA.
发表日期	2024
ISSN	0027-8424
EISSN	1091-6490
起始页码	121
结束页码	6
卷号	121 期号:6
英文摘要	Coastal Antarctic marine ecosystems are significant in carbon cycling because of their intense seasonal phytoplankton blooms. Southern Ocean algae are primarily limited by light and iron (Fe) and can be co -limited by cobalamin (vitamin B12). Micronutrient limitation controls productivity and shapes the composition of blooms which are typically dominated by either diatoms or the haptophyte Phaeocystis antarctica. However, the vitamin requirements and ecophysiology of the keystone species P. antarctica remain poorly characterized. Using cultures, physiological analysis, and comparative omics, we examined the response of P. antarctica to a matrix of Fe -B12 conditions. We show that P. antarctica is not auxotrophic for B12, as previously suggested, and identify mechanisms underlying its B12 response in cultures of predominantly solitary and colonial cells. A combination of proteomics and proteogenomics reveals a B12 -independent methionine synthase fusion protein (MetE-fusion) that is expressed under vitamin limitation and interreplaced with the B12 -dependent isoform under replete conditions. Database searches return homologues of the MetE-fusion protein in multiple Phaeocystis species and in a wide range of marine microbes, including other photosynthetic eukaryotes with polymorphic life cycles as well as bacterioplankton. Furthermore, we find MetE-fusion homologues expressed in metaproteomic and metatranscriptomic field samples in polar and more geographically widespread regions. As climate change impacts micronutrient availability in the coastal Southern Ocean, our finding that P. antarctica has a flexible B12 metabolism has implications for its relative fitness compared to B12-auxotrophic diatoms and for the detection of B12 -stress in a more diverse set of marine microbes.
英文关键词	Phaeocystis antarctica; iron; proteogenomics; methionine synthase; B12
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:001167423500002
来源期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/299324
作者单位	Massachusetts Institute of Technology (MIT); J. Craig Venter Institute; University of California System; University of California San Diego; Scripps Institution of Oceanography
推荐引用方式 GB/T 7714	Rao, Deepa,Fussy, Zoltan,Brisbin, Margaret M.,et al. Flexible B12 ecophysiology of Phaeocystis antarctica due to a fusion B12-independent methionine synthase with widespread homologues[J],2024,121(6).
APA	Rao, Deepa.,Fussy, Zoltan.,Brisbin, Margaret M..,McIlvin, Matthew R..,Moran, Dawn M..,...&Saito, MakA..(2024).Flexible B12 ecophysiology of Phaeocystis antarctica due to a fusion B12-independent methionine synthase with widespread homologues.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,121(6).
MLA	Rao, Deepa,et al."Flexible B12 ecophysiology of Phaeocystis antarctica due to a fusion B12-independent methionine synthase with widespread homologues".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 121.6(2024).