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DOI	10.1016/j.jep.2024.117933
	The whitening effect of cuscutin responsible for traditional use of Bergenia purpurascens
	Liu, Yang-Yang; Zhang, Yue; Jiang, Ling; Lu, Qing-Yu; Ye, Rui-Han; Guo, Zhen-Yu; Zhao, Yun-Li; Luo, Xiao-Dong
发表日期	2024
ISSN	0378-8741
EISSN	1872-7573
起始页码	326
卷号	326
英文摘要	Ethnopharmacological relevance: The roots and rhizomes of Bergenia purpurascens (Hook. f. et Thomson) Engl., was used as a sunscreen to protect against ultraviolet rays in Tibet of China historically, but its skin whitening constituents and pharmacological effects of this plant remained unknown. Aim of the study: To investigate the anti-melanogenesis effect of B. purpurascens in vitro and in vivo, and then explore the preliminary mechanism. Materials and methods: An ultraviolet B (UVB)-induced skin injury model of mice was used to verify the ameliorative effect of B. purpurascens extract (BPE) on ultraviolet damage. Then, alpha-melanocyte stimulating hormone (alpha-MSH)-induced murine melanoma cell line (B16F10) melanin generation model was further adopted to approval the effects of BPE and its bioactive compound, cuscutin, in vitro. Moreover, alpha-MSH stimulated melanogenesis model in zebrafish was employed to confirm the anti-pigmentation effect of cuscutin. Then, proteins expressions associated with melanin production were observed using western blotting assay to explore preliminary mechanism. Results: BPE inhibited UVB-induced mice injury and restored skin barrier function observably in vivo. BPE and cuscutin suppressed the overproduction of melanin in alpha-MSH induced B16F10 significantly, in which cuscutin exhibited better effect than well-known whitening agent alpha-arbutin at same 10 mu g/mL concentration. Moreover, the pigmentation of zebrafish embryo was decreased by cuscutin. Finally, cuscutin showed significant downregulation of expressions of tyrosinase (TYR) and tyrosinase related protein-1 (TRP-1), TRP-2 and microphthalmia-associated transcription factor (MITF) in the melanogenic signaling pathway. Conclusion: B. purpurascens extract and its major bioactive constituent, cuscutin, showed potent antimelanogenesis and skin-whitening effect by targeting TYR and TRP-2 proteins for the first time, which supported its traditional use.
英文关键词	Bergenia purpurascens; Cuscutin; Whitening; Anti-melanogenesis
语种	英语
WOS研究方向	Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
WOS类目	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
WOS记录号	WOS:001203022600001
来源期刊	JOURNAL OF ETHNOPHARMACOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/299078
作者单位	Yunnan University; Chinese Academy of Sciences; Kunming Institute of Botany, CAS
推荐引用方式 GB/T 7714	Liu, Yang-Yang,Zhang, Yue,Jiang, Ling,et al. The whitening effect of cuscutin responsible for traditional use of Bergenia purpurascens[J],2024,326.
APA	Liu, Yang-Yang.,Zhang, Yue.,Jiang, Ling.,Lu, Qing-Yu.,Ye, Rui-Han.,...&Luo, Xiao-Dong.(2024).The whitening effect of cuscutin responsible for traditional use of Bergenia purpurascens.JOURNAL OF ETHNOPHARMACOLOGY,326.
MLA	Liu, Yang-Yang,et al."The whitening effect of cuscutin responsible for traditional use of Bergenia purpurascens".JOURNAL OF ETHNOPHARMACOLOGY 326(2024).