气候变化领域集成服务门户(CCPortal): Comparison of Taxonomic Resolutions of Various Typing Methods for Clostridium and Paraclostridium Species Isolated from Landfill Leachate

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DOI	10.1007/s12088-023-01179-1
	Comparison of Taxonomic Resolutions of Various Typing Methods for Clostridium and Paraclostridium Species Isolated from Landfill Leachate
	Nimonkar, Yogesh S.; Ranade, Dilip R.; Poojary, Aruna; Dake, Manjusha S.; Prakash, Om
发表日期	2024
ISSN	0046-8991
EISSN	0973-7715
英文摘要	Anaerobic bacteria and methanogenic archaea at municipal landfill dumping sites play a vital role in the landfill ecology, waste degradation, global warming and climate change. Although landfill works as a cheap way of solid waste management, unmanaged landfill plays a significant role in spreading pollutants and pathogens in natural ecosystems. The genera Clostridium and Paraclostridium are important groups of anaerobic microbes from a public and environmental health perspective. In the present study, we have isolated different species of Clostridium and Paraclostridium from landfill leachate and performed a comparative analysis to understand their role in landfill ecology and public health. Our data indicate that the anoxic zone of landfills acts as a breeding ground for different species of Clostridium and Paraclostridium, including pathogenic species like C. sporogenes and C. argentinense and leachate, plays a crucial role in the spread of Clostridium in soil and water ecosystem and acts as one of the primary sources of a cause of infection in field workers. We also reported that VITEK and MALDI-TOF/MS are not ideal ways to identify Clostridium at the species level, and 16S rRNA gene sequencing should be the method of choice. In the current study, we also demonstrated that members of the genus Clostridium showed better growth response on Brain Heart Infusion (BHI) and Gifu Anaerobic Medium (GAM), and they can be one of the alternatives to existing media for cultivation and physiological studies of Clostridium.
英文关键词	Landfill; Leachate; Clostridium; Paraclostridium; Contamination
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:001135608500002
来源期刊	INDIAN JOURNAL OF MICROBIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/298438
作者单位	Department of Biotechnology (DBT) India; National Centre for Cell Science, Pune (NCCS); Symbiosis International University
推荐引用方式 GB/T 7714	Nimonkar, Yogesh S.,Ranade, Dilip R.,Poojary, Aruna,et al. Comparison of Taxonomic Resolutions of Various Typing Methods for Clostridium and Paraclostridium Species Isolated from Landfill Leachate[J],2024.
APA	Nimonkar, Yogesh S.,Ranade, Dilip R.,Poojary, Aruna,Dake, Manjusha S.,&Prakash, Om.(2024).Comparison of Taxonomic Resolutions of Various Typing Methods for Clostridium and Paraclostridium Species Isolated from Landfill Leachate.INDIAN JOURNAL OF MICROBIOLOGY.
MLA	Nimonkar, Yogesh S.,et al."Comparison of Taxonomic Resolutions of Various Typing Methods for Clostridium and Paraclostridium Species Isolated from Landfill Leachate".INDIAN JOURNAL OF MICROBIOLOGY (2024).