气候变化领域集成服务门户(CCPortal): Transcriptome variation in banded newt (Ommatotriton vittatus) during its life cycle and habitat transition

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DOI	10.1016/j.cbd.2024.101203
	Transcriptome variation in banded newt (Ommatotriton vittatus) during its life cycle and habitat transition
	Degani, Gad; Meerson, Ari
发表日期	2024
ISSN	1744-117X
EISSN	1878-0407
起始页码	50
卷号	50
英文摘要	Israel represents the southern limit of the distribution of the banded newt (Ommatotriton vittatus). The life cycle of O. vittatus includes several distinct phases: eggs, aquatic larvae, a terrestrial phase and an aquatic reproductive phase. We investigated differences in gene expression during the life cycle and transition of banded newts between terrestrial and aquatic habitats using mRNA-seq. We identified -10 k genes that were differentially expressed (DE) in one of the pairwise comparisons between 3 groups: 1 - terrestrial newts (males and females), 2aquatic newts (males and females), 3 - aquatic larvae before metamorphosis. The groups were clearly defined by Principal Components Analysis (PCA). The greatest difference was between aquatic newts (males and females) and aquatic larvae: -7.4 k DE genes. Of special interest were the -2.4 k genes DE between the aquatic and terrestrial phenotypes. These included prominent candidates with known roles in kidney function (uromodulin homologs were strongly associated with aquatic lifestyle), tissue structure (keratins), and the thyroid hormone signaling modulator DUOXA1. Additional developmental and metabolic pathways overrepresented among the identified DE genes included epidermis development, nervous system development, nucleotide-sugar biosynthesis. Overall, both metamorphosis and environmental transition of banded newts involve extensive transcriptomic remodeling involving developmental, metabolic, and cellular pathways. Understanding the roles of these pathways and individual genes is instrumental for studies of transition between habitats, especially those affected by climate change. Furthermore, the phenotypic flexibility of the newt and the underlying regulation of gene expression can shed light on the evolution of terrestrial vertebrates.
英文关键词	Banded newt; Amphibians; Life cycle; Metamorphosis; Environmental transition; Transcriptome; Gene expression; mRNA-seq
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS类目	Biochemistry & Molecular Biology ; Genetics & Heredity
WOS记录号	WOS:001180815300001
来源期刊	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/298284
作者单位	Tel Hai Academic College
推荐引用方式 GB/T 7714	Degani, Gad,Meerson, Ari. Transcriptome variation in banded newt (Ommatotriton vittatus) during its life cycle and habitat transition[J],2024,50.
APA	Degani, Gad,&Meerson, Ari.(2024).Transcriptome variation in banded newt (Ommatotriton vittatus) during its life cycle and habitat transition.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,50.
MLA	Degani, Gad,et al."Transcriptome variation in banded newt (Ommatotriton vittatus) during its life cycle and habitat transition".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 50(2024).