气候变化领域集成服务门户(CCPortal): Integrated analysis of transcriptomic and physiological responses to cold stress in Macrobrachium rosenbergii

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DOI	10.1016/j.aqrep.2024.102042
	Integrated analysis of transcriptomic and physiological responses to cold stress in Macrobrachium rosenbergii
	Yu, Jiongying; Yi, Shaokui; Yang, Guoliang; Wang, Weimin
发表日期	2024
ISSN	2352-5134
起始页码	36
卷号	36
英文摘要	The giant freshwater prawn (GFP), Macrobrachium rosenbergii , stands out as a vital species in freshwater shrimp aquaculture. However, its susceptibility to cold conditions has increased the cost of aquaculture and limited the growth of GFP cultivation. To furnish insights at the molecular level to enhance GFP ' s cold tolerance, transcriptome sequencing and physiological analysis of the hepatopancreas were performed in our study. Initially, we identified 4909 differentially expressed genes (DEGs) between experimental (15 degrees C, 24 h) and control groups (25 degrees C, 24 h). These DEGs were significantly enriched in the proteasome, spliceosome, lysosome, peroxisome, and lipid metabolism-related pathways including glycerolipid metabolism, sphingolipid metabolism, linoleic acid metabolism as well as steroid hormone biosynthesis. Histological examination following various stress durations revealed a notable increase in hepatocyte vacuoles after 6 h of cold stress. Hepatocyte structure deterioration occurred in the later phase (48 h), coinciding with a significant increase in cell apoptosis. Subsequent analyses demonstrated an initial increase followed by a decrease in superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) content, while malonaldehyde (MDA) content rose with stress duration. Furthermore, a significant increase in triglyceride content and no significant change in cholesterol content was observed. RT-qPCR results unveiled significant down-regulated expression of FAS , TAGL , ACOX1 , ACS and significant up-regulated expression of GPAT and DEGS . These findings suggest that oxidative stress and impaired lipid metabolism underlie the poor cold tolerance of GFP.
英文关键词	Macrobrachium rosenbergii; Cold stress; Hepatopancreas; Oxidative stress; Lipid metabolisms
语种	英语
WOS研究方向	Fisheries
WOS类目	Fisheries
WOS记录号	WOS:001216696100001
来源期刊	AQUACULTURE REPORTS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/297943
作者单位	Huazhong Agricultural University; Huzhou University; Huazhong Agricultural University
推荐引用方式 GB/T 7714	Yu, Jiongying,Yi, Shaokui,Yang, Guoliang,et al. Integrated analysis of transcriptomic and physiological responses to cold stress in Macrobrachium rosenbergii[J],2024,36.
APA	Yu, Jiongying,Yi, Shaokui,Yang, Guoliang,&Wang, Weimin.(2024).Integrated analysis of transcriptomic and physiological responses to cold stress in Macrobrachium rosenbergii.AQUACULTURE REPORTS,36.
MLA	Yu, Jiongying,et al."Integrated analysis of transcriptomic and physiological responses to cold stress in Macrobrachium rosenbergii".AQUACULTURE REPORTS 36(2024).