气候变化领域集成服务门户(CCPortal): Liquid in vitro culture system allows gradual intensification of osmotic stress in Solanum tuberosum through sorbitol

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DOI	10.1007/s11240-024-02720-w
	Liquid in vitro culture system allows gradual intensification of osmotic stress in Solanum tuberosum through sorbitol
	Wellpott, Katharina; Herde, Marco; Winkelmann, Traud; Buendig, Christin
发表日期	2024
ISSN	0167-6857
EISSN	1573-5044
起始页码	157
结束页码	1
卷号	157 期号:1
英文摘要	Because of their shallow root system, drought stress is a major problem in potato cultivation. Due to climate change more severe drought periods are expected to occur in the vegetative growth phase of potato growth. Therefore, there is a great need for drought tolerant potato genotypes. Potato responds to drought stress in the field in various ways, including osmoregulation. Osmotic stress can be induced in vitro by adding an osmotic agent and thus lowering the osmotic potential of the medium. In this study, a new, cost-effective in vitro test system is presented, in which the osmotic agent can be gradually added after root formation to prevent an osmotic shock. This is achieved by using sieves as plant holders and liquid medium, which, allows an improved simulation of gradually drying soil. Responses to osmotic stress in four potato genotypes were analysed and an increase in proline under osmotic stress was detected. Moreover, genes of interest that were postulated to be linked to drought stress were shown by quantitative qRT-PCR to be regulated under osmotic stress. Furthermore, we showed that the content of sorbitol, which was used as osmotic agent, was 700- fold higher for 'Eurostarch' after seven days under osmotic stress and 1093- fold higher after 14 days, respectively, compared to control plants without sorbitol addition. Therefore, further investigations must show, whether it was taken up through the roots, is metabolised, stored or de novo synthesised by the potato plants.
英文关键词	Osmotic stress; Gene expression; In vitro test system; Sorbitol uptake
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS记录号	WOS:001197274500003
来源期刊	PLANT CELL TISSUE AND ORGAN CULTURE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/297755
作者单位	Leibniz University Hannover; Leibniz University Hannover
推荐引用方式 GB/T 7714	Wellpott, Katharina,Herde, Marco,Winkelmann, Traud,et al. Liquid in vitro culture system allows gradual intensification of osmotic stress in Solanum tuberosum through sorbitol[J],2024,157(1).
APA	Wellpott, Katharina,Herde, Marco,Winkelmann, Traud,&Buendig, Christin.(2024).Liquid in vitro culture system allows gradual intensification of osmotic stress in Solanum tuberosum through sorbitol.PLANT CELL TISSUE AND ORGAN CULTURE,157(1).
MLA	Wellpott, Katharina,et al."Liquid in vitro culture system allows gradual intensification of osmotic stress in Solanum tuberosum through sorbitol".PLANT CELL TISSUE AND ORGAN CULTURE 157.1(2024).