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| DOI | 10.1007/s13237-024-00490-6 |
| Identification and validation of defense related candidate genes in Sesamum under artificial inoculation of Macrophomina phaseolina | |
| 发表日期 | 2024 |
| ISSN | 0029-568X |
| EISSN | 0976-7975 |
| 英文摘要 | Sesame (Sesamum indicum L.) suffers significant production loss due to the opportunistic fungus Macrophomina phaseolina which causes charcoal rot. Climate change-induced increasing temperatures and irregular seasonal patterns create the propitious disease triangle necessary for the infestation of this destructive fungus. This demands an improved sesame genotype for enhanced charcoal rot tolerance, achieved through our novel inter-specific hybrid (S. indicum x S. mulayanum). To get a global overview of transcripts responsive to induced infection of M. phaseolina, RNA-sequencing of cultivated (S. indicum), wild (S. mulayanum) and inter-specific hybrid was performed. Several potential differentially expressed genes (DEGs) which could foster an understanding of the Mp-sesame-pathosystem, were subjected to fluorescence-based quantitative real-time PCR (RT-qPCR). Potential candidate genes were annotated with model plant Arabidopsis to find putative functions based on conserved sequence similarity. Bioinformatics analysis was performed to gain insights into cellular localization, isoelectric point and protein molecular weight of identified candidate genes. Our combined results of the disease susceptibility index, and expression analysis of candidate genes highlighted the role of cell wall-related genes, phenylpropanoid pathway, hydrolases and expression trends in inter-specific hybrid, suggested enhanced tolerance to charcoal rot. Our study findings would bolster the understanding of the defense mechanism of sesame in response to M. phaseolina. |
| 英文关键词 | Sesame; Macrophomina infection; Transcriptomic data; Defense genes; Validation |
| 语种 | 英语 |
| WOS研究方向 | Cell Biology |
| WOS类目 | Cell Biology |
| WOS记录号 | WOS:001205907000001 |
| 来源期刊 | NUCLEUS-INDIA
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/297630 |
| 作者单位 | Department of Science & Technology (India); Bose Institute |
| 推荐引用方式 GB/T 7714 | . Identification and validation of defense related candidate genes in Sesamum under artificial inoculation of Macrophomina phaseolina[J],2024. |
| APA | (2024).Identification and validation of defense related candidate genes in Sesamum under artificial inoculation of Macrophomina phaseolina.NUCLEUS-INDIA. |
| MLA | "Identification and validation of defense related candidate genes in Sesamum under artificial inoculation of Macrophomina phaseolina".NUCLEUS-INDIA (2024). |
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