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| DOI | 10.1063/5.0193920 |
| Dielectrophoretic-inertial microfluidics for Symbiodinium separation and enrichment | |
| Zhou, Teng; He, Jixin; Wu, Zhihao; Bian, Qin; He, Xiaohan; Zhou, Shizheng; Zhao, Juncheng; Wu, Tao; Shi, Liuyong; Yan, Hong | |
| 发表日期 | 2024 |
| ISSN | 1070-6631 |
| EISSN | 1089-7666 |
| 起始页码 | 36 |
| 结束页码 | 3 |
| 卷号 | 36期号:3 |
| 英文摘要 | In the marine environment, the symbiotic relationship between Symbiodinium and corals plays a pivotal role in coral growth and development. Against the backdrop of widespread coral bleaching due to the global climate change, the facile and efficient separation and enrichment of different strains of Symbiodinium hold significant importance for studying coral bleaching. This paper aims to report a platform that integrates dielectrophoretic and inertial forces for the separation and enrichment of Symbiodinium, comprising two modular components: a separation module and an enrichment module. Within the separation module, distinct strains of Symbiodinium undergo preliminary stratification in a contraction-expansion microchannel under the influence of inertial forces. Dielectrophoretic forces generated by the indium tin oxide electrodes divert them toward different outlets, achieving separation. In the enrichment module, the Symbiodinium collected from outlets is rapidly focused through a contraction-expansion microchannel and high-purity samples are concentrated through a single outlet. Evaluating separation efficiency is based on the purity of collected Symbiodinium at the outlet under three different flow rates: 13, 16, and 19 mu l/min, while the concentration of enriched Symbiodinium at 100, 200, 300, and 400 mu l/min flow rates evaluates the effectiveness of the enrichment process. The experimental results demonstrate a separation purity of approximately 90% and an enrichment factor of around 5.5. The platform holds promise for further applications in the selection and targeted enrichment of high-quality coral symbiotic algae, providing essential research foundations for the conservation of coral ecosystems. |
| 语种 | 英语 |
| WOS研究方向 | Mechanics ; Physics |
| WOS类目 | Mechanics ; Physics, Fluids & Plasmas |
| WOS记录号 | WOS:001183702100019 |
| 来源期刊 | PHYSICS OF FLUIDS
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/297128 |
| 作者单位 | Hainan University |
| 推荐引用方式 GB/T 7714 | Zhou, Teng,He, Jixin,Wu, Zhihao,et al. Dielectrophoretic-inertial microfluidics for Symbiodinium separation and enrichment[J],2024,36(3). |
| APA | Zhou, Teng.,He, Jixin.,Wu, Zhihao.,Bian, Qin.,He, Xiaohan.,...&Yan, Hong.(2024).Dielectrophoretic-inertial microfluidics for Symbiodinium separation and enrichment.PHYSICS OF FLUIDS,36(3). |
| MLA | Zhou, Teng,et al."Dielectrophoretic-inertial microfluidics for Symbiodinium separation and enrichment".PHYSICS OF FLUIDS 36.3(2024). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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