Climate Change Data Portal
| DOI | 10.1021/jacsau.3c00696 |
| Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7 | |
| 发表日期 | 2024 |
| EISSN | 2691-3704 |
| 起始页码 | 4 |
| 结束页码 | 2 |
| 卷号 | 4期号:2 |
| 英文摘要 | In the era of global climate change, the increasingly severe Fusarium head blight (FHB) and deoxynivalenol (DON) contamination have caused economic losses and brought food and feed safety concerns. Recently, an FHB resistance gene Fhb7 coding a glutathione-S transferase (GST) to degrade DON by opening the critical toxic epoxide moiety was identified and opened a new window for wheat breeding and DON detoxification. However, the poor stability of Fhb7 and the elusiveness of the catalytic mechanism hinder its practical application. Herein, we report the first structure of Fhb7 at 2.41 angstrom and reveal a unique catalytic mechanism of epoxide opening transformation in GST family proteins. Furthermore, variants V29P and M10 showed that 5.5-fold and 266.7-fold longer half-life time than wild-type, respectively, were identified. These variants offer broad substrate scope, and the engineered biosafe Bacillus subtilis overexpressing the variants shows excellent DON degradation performance, exhibiting potential at bacterium engineering to achieve DON detoxification in the feed and biomedicine industry. This work provides a profound mechanistic insight into the enzymatic activities of Fhb7 and paves the way for further utilizing Fhb7-related enzymes in crop breeding and DON detoxification by synthetic biology. |
| 英文关键词 | deoxynivalenol degradation; Fhb7; thermal stability; consensus sequence; protein engineering; syntheticbiology; Fusarium head blight |
| 语种 | 英语 |
| WOS研究方向 | Chemistry |
| WOS类目 | Chemistry, Multidisciplinary |
| WOS记录号 | WOS:001164561300001 |
| 来源期刊 | JACS AU
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/295310 |
| 作者单位 | Peking University; Peking University; Peking University; Peking University; Chinese Academy of Sciences; Dalian Institute of Chemical Physics, CAS; Chinese Academy of Sciences; Shanghai Institute of Materia Medica, CAS; Chinese Academy of Sciences; University of Chinese Academy of Sciences, CAS; Shenzhen Bay Laboratory |
| 推荐引用方式 GB/T 7714 | . Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7[J],2024,4(2). |
| APA | (2024).Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7.JACS AU,4(2). |
| MLA | "Enzymatic Degradation of Deoxynivalenol with the Engineered Detoxification Enzyme Fhb7".JACS AU 4.2(2024). |
| 条目包含的文件 | 条目无相关文件。 | |||||
| 个性服务 |
| 推荐该条目 |
| 保存到收藏夹 |
| 导出为Endnote文件 |
| 谷歌学术 |
| 谷歌学术中相似的文章 |
| 百度学术 |
| 百度学术中相似的文章 |
| 必应学术 |
| 必应学术中相似的文章 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
