气候变化领域集成服务门户(CCPortal): Phenylboronic Acid-Functionalized Micelles Dual-Targeting Boronic Acid Transporter and Polysaccharides for siRNA Delivery into Brown Algae

CCPortal

DOI	10.1021/jacsau.3c00767
	Phenylboronic Acid-Functionalized Micelles Dual-Targeting Boronic Acid Transporter and Polysaccharides for siRNA Delivery into Brown Algae
	Yoshinaga, Naoto; Miyamoto, Takaaki; Goto, Mami; Tanaka, Atsuko; Numata, Keiji
发表日期	2024
EISSN	2691-3704
起始页码	4
结束页码	4
卷号	4 期号:4
英文摘要	Brown algae play essential roles ecologically, practically, and evolutionarily because they maintain coastal areas, capture carbon dioxide, and produce valuable chemicals such as therapeutic drugs. To unlock their full potential, understanding the unique molecular biology of brown algae is imperative. Genetic engineering tools that regulate homeostasis in brown algae are essential for determining their biological mechanisms in detail. However, few methodologies have been developed to control gene expression due to the robust structural barriers of brown algae. To address this issue, we designed peptide-based, small interfering RNA (siRNA)-loaded micelles decorated with phenylboronic acid (PBA) ligands. The PBA ligands facilitated the cellular uptake of the micelles into a model brown alga, Ectocarpus siliculosus (E. Siliculosus), through chemical interaction with polysaccharides in the cell wall and biological recognition by boronic acid transporters on the plasma membrane. The micelles, featuring kill two birds with one stone ligands, effectively induced gene silencing related to auxin biosynthesis. As a result, the growth of E. siliculosus was temporarily inhibited without persistent genome editing. This study demonstrated the potential for exploring the characteristics of brown algae through a simple yet effective approach and presented a feasible system for delivering siRNA in brown algae.
英文关键词	brown algae; siRNA; peptide; phenylboronicacid; micelles; auxin biosynthesis
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:001189969200001
来源期刊	JACS AU
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/295295
作者单位	RIKEN; Keio University; Kyoto University; University of the Ryukyus
推荐引用方式 GB/T 7714	Yoshinaga, Naoto,Miyamoto, Takaaki,Goto, Mami,et al. Phenylboronic Acid-Functionalized Micelles Dual-Targeting Boronic Acid Transporter and Polysaccharides for siRNA Delivery into Brown Algae[J],2024,4(4).
APA	Yoshinaga, Naoto,Miyamoto, Takaaki,Goto, Mami,Tanaka, Atsuko,&Numata, Keiji.(2024).Phenylboronic Acid-Functionalized Micelles Dual-Targeting Boronic Acid Transporter and Polysaccharides for siRNA Delivery into Brown Algae.JACS AU,4(4).
MLA	Yoshinaga, Naoto,et al."Phenylboronic Acid-Functionalized Micelles Dual-Targeting Boronic Acid Transporter and Polysaccharides for siRNA Delivery into Brown Algae".JACS AU 4.4(2024).