气候变化领域集成服务门户(CCPortal): Host genotype-specific rhizosphere fungus enhances drought resistance in wheat

CCPortal

DOI	10.1186/s40168-024-01770-8
	Host genotype-specific rhizosphere fungus enhances drought resistance in wheat
	Yue, Hong; Sun, Xuming; Wang, Tingting; Zhang, Ali; Han, Dejun; Wei, Gehong; Song, Weining; Shu, Duntao
发表日期	2024
ISSN	2049-2618
起始页码	12
结束页码	1
卷号	12 期号:1
英文摘要	BackgroundThe severity and frequency of drought are expected to increase substantially in the coming century and dramatically reduce crop yields. Manipulation of rhizosphere microbiomes is an emerging strategy for mitigating drought stress in agroecosystems. However, little is known about the mechanisms underlying how drought-resistant plant recruitment of specific rhizosphere fungi enhances drought adaptation of drought-sensitive wheats. Here, we investigated microbial community assembly features and functional profiles of rhizosphere microbiomes related to drought-resistant and drought-sensitive wheats by amplicon and shotgun metagenome sequencing techniques. We then established evident linkages between root morphology traits and putative keystone taxa based on microbial inoculation experiments. Furthermore, root RNA sequencing and RT-qPCR were employed to explore the mechanisms how rhizosphere microbes modify plant response traits to drought stresses.ResultsOur results indicated that host plant signature, plant niche compartment, and planting site jointly contribute to the variation of soil microbiome assembly and functional adaptation, with a relatively greater effect of host plant signature observed for the rhizosphere fungi community. Importantly, drought-resistant wheat (Yunhan 618) possessed more diverse bacterial and fungal taxa than that of the drought-sensitive wheat (Chinese Spring), particularly for specific fungal species. In terms of microbial interkingdom association networks, the drought-resistant variety possessed more complex microbial networks. Metagenomics analyses further suggested that the enriched rhizosphere microbiomes belonging to the drought-resistant cultivar had a higher investment in energy metabolism, particularly in carbon cycling, that shaped their distinctive drought tolerance via the mediation of drought-induced feedback functional pathways. Furthermore, we observed that host plant signature drives the differentiation in the ecological role of the cultivable fungal species Mortierella alpine (M. alpina) and Epicoccum nigrum (E. nigrum). The successful colonization of M. alpina on the root surface enhanced the resistance of wheats in response to drought stresses via activation of drought-responsive genes (e.g., CIPK9 and PP2C30). Notably, we found that lateral roots and root hairs were significantly suppressed by co-colonization of a drought-enriched fungus (M. alpina) and a drought-depleted fungus (E. nigrum).ConclusionsCollectively, our findings revealed host genotypes profoundly influence rhizosphere microbiome assembly and functional adaptation, as well as it provides evidence that drought-resistant plant recruitment of specific rhizosphere fungi enhances drought tolerance of drought-sensitive wheats. These findings significantly underpin our understanding of the complex feedbacks between plants and microbes during drought, and lay a foundation for steering beneficial keystone biome to develop more resilient and productive crops under climate change.FGv_ocsEwwAXXw4e-N4VhrVideo AbstractConclusionsCollectively, our findings revealed host genotypes profoundly influence rhizosphere microbiome assembly and functional adaptation, as well as it provides evidence that drought-resistant plant recruitment of specific rhizosphere fungi enhances drought tolerance of drought-sensitive wheats. These findings significantly underpin our understanding of the complex feedbacks between plants and microbes during drought, and lay a foundation for steering beneficial keystone biome to develop more resilient and productive crops under climate change.FGv_ocsEwwAXXw4e-N4VhrVideo Abstract
英文关键词	Wheat; Rhizosphere; Microbiome; Drought; Multi-omics
语种	英语
WOS研究方向	Microbiology
WOS类目	Microbiology
WOS记录号	WOS:001177124700001
来源期刊	MICROBIOME
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/294976
作者单位	Northwest A&F University - China; Northwest A&F University - China
推荐引用方式 GB/T 7714	Yue, Hong,Sun, Xuming,Wang, Tingting,et al. Host genotype-specific rhizosphere fungus enhances drought resistance in wheat[J],2024,12(1).
APA	Yue, Hong.,Sun, Xuming.,Wang, Tingting.,Zhang, Ali.,Han, Dejun.,...&Shu, Duntao.(2024).Host genotype-specific rhizosphere fungus enhances drought resistance in wheat.MICROBIOME,12(1).
MLA	Yue, Hong,et al."Host genotype-specific rhizosphere fungus enhances drought resistance in wheat".MICROBIOME 12.1(2024).