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DOI | 10.1186/s12863-024-01198-x |
Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling | |
Costes, Camille; Navarro Sanz, Sergi; Calatayud, Caroline; Soriano, Alexandre; Mameri, Hamza; Terrier, Nancy; Francin-Allami, Mathilde | |
发表日期 | 2024 |
EISSN | 2730-6844 |
起始页码 | 25 |
结束页码 | 1 |
卷号 | 25期号:1 |
英文摘要 | ObjectiveSorghum (Sorghum bicolor (L.) Moench) is the fifth most important grain produced in the world. Interest for cultivating sorghum is increasing all over the world in the context of climate change, due to its low input and water requirements. Like other cultivated cereals, sorghum has significant nutritional value thanks to its protein, carbohydrate and dietary fiber content, these latter mainly consisting of cell wall polysaccharides. This work describes for the first time a transcriptomic analysis dedicated to identify the genes involved in the biosynthesis and remodelling of cell walls both in the endosperm and outer layers of sorghum grain during its development. Further analysis of these transcriptomic data will improve our understanding of cell wall assembly, which is a key component of grain quality.Data descriptionThis research delineates the steps of our analysis, starting with the cultivation conditions and the grain harvest at different stages of development, followed by the laser microdissection applied to separate the endosperm from the outer layers. It also describes the procedures implemented to generate RNA libraries and to obtain a normalized and filtered table of transcript counts, and finally determine the number of putative cell wall-related genes already listed in literature. |
英文关键词 | Sorghum; Grain development; Laser microdissection; Transcriptome; Cell wall polysaccharides |
语种 | 英语 |
WOS研究方向 | Genetics & Heredity |
WOS类目 | Genetics & Heredity |
WOS记录号 | WOS:001157196700001 |
来源期刊 | BMC GENOMIC DATA |
文献类型 | 期刊论文 |
条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/294269 |
作者单位 | INRAE; Universite de Montpellier; CIRAD; Institut Agro; Universite de Montpellier; CIRAD; Centre National de la Recherche Scientifique (CNRS); Institut National de la Sante et de la Recherche Medicale (Inserm); Universite de Montpellier; INRAE; Institut Agro; Universite de Montpellier |
推荐引用方式 GB/T 7714 | Costes, Camille,Navarro Sanz, Sergi,Calatayud, Caroline,et al. Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling[J],2024,25(1). |
APA | Costes, Camille.,Navarro Sanz, Sergi.,Calatayud, Caroline.,Soriano, Alexandre.,Mameri, Hamza.,...&Francin-Allami, Mathilde.(2024).Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling.BMC GENOMIC DATA,25(1). |
MLA | Costes, Camille,et al."Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling".BMC GENOMIC DATA 25.1(2024). |
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