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DOI10.1186/s12863-024-01198-x
Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling
Costes, Camille; Navarro Sanz, Sergi; Calatayud, Caroline; Soriano, Alexandre; Mameri, Hamza; Terrier, Nancy; Francin-Allami, Mathilde
发表日期2024
EISSN2730-6844
起始页码25
结束页码1
卷号25期号:1
英文摘要ObjectiveSorghum (Sorghum bicolor (L.) Moench) is the fifth most important grain produced in the world. Interest for cultivating sorghum is increasing all over the world in the context of climate change, due to its low input and water requirements. Like other cultivated cereals, sorghum has significant nutritional value thanks to its protein, carbohydrate and dietary fiber content, these latter mainly consisting of cell wall polysaccharides. This work describes for the first time a transcriptomic analysis dedicated to identify the genes involved in the biosynthesis and remodelling of cell walls both in the endosperm and outer layers of sorghum grain during its development. Further analysis of these transcriptomic data will improve our understanding of cell wall assembly, which is a key component of grain quality.Data descriptionThis research delineates the steps of our analysis, starting with the cultivation conditions and the grain harvest at different stages of development, followed by the laser microdissection applied to separate the endosperm from the outer layers. It also describes the procedures implemented to generate RNA libraries and to obtain a normalized and filtered table of transcript counts, and finally determine the number of putative cell wall-related genes already listed in literature.
英文关键词Sorghum; Grain development; Laser microdissection; Transcriptome; Cell wall polysaccharides
语种英语
WOS研究方向Genetics & Heredity
WOS类目Genetics & Heredity
WOS记录号WOS:001157196700001
来源期刊BMC GENOMIC DATA
文献类型期刊论文
条目标识符http://gcip.llas.ac.cn/handle/2XKMVOVA/294269
作者单位INRAE; Universite de Montpellier; CIRAD; Institut Agro; Universite de Montpellier; CIRAD; Centre National de la Recherche Scientifique (CNRS); Institut National de la Sante et de la Recherche Medicale (Inserm); Universite de Montpellier; INRAE; Institut Agro; Universite de Montpellier
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GB/T 7714
Costes, Camille,Navarro Sanz, Sergi,Calatayud, Caroline,et al. Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling[J],2024,25(1).
APA Costes, Camille.,Navarro Sanz, Sergi.,Calatayud, Caroline.,Soriano, Alexandre.,Mameri, Hamza.,...&Francin-Allami, Mathilde.(2024).Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling.BMC GENOMIC DATA,25(1).
MLA Costes, Camille,et al."Transcriptomic analysis of developing sorghum grains to detect genes related to cell wall biosynthesis and remodelling".BMC GENOMIC DATA 25.1(2024).
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