Climate Change Data Portal
| DOI | 10.1016/j.algal.2024.103487 |
| Heterologous assembly of red-type Rubisco and functional identification of chaperonin in Porphyridium purpureum | |
| 发表日期 | 2024 |
| ISSN | 2211-9264 |
| 起始页码 | 79 |
| 卷号 | 79 |
| 英文摘要 | In the natural growth process of organisms, Ribulose 1,5-diphosphate carboxylase/oxygenase (Rubisco) is the rate-limiting enzyme for carbon fixation in photosynthesis. However, its carboxylation is low and the reaction can be competitively inhibited by O 2 . Red-type Rubiscos are known with higher catalytic rates and CO 2 /O 2 specificity, but their incompatibility with chaperonins in the green lineage pose challenges for them to be assembled and accumulated in C3 plants. In this study, we systematically explored the factors and conditions required for heterologous accumulation Rubisco from Porphyridium purpureum and that from Phaeodactylum tricornutum . We found that P. purpureum Rubisco required less co-factors to be produced in Escherichia coli than the P. tricornutum needed. In addition, the amino acid sequences of the co-chaperonin Cpn10 and Cpn20 in P. purpureum were retrieved, and they can assist in GroEL to fold the red-type Rubisco large subunits in vitro and in E. coli . Heterogeneous system research on Rubisco assembly conditions are of great significance for humans to understand CO 2 fixation mechanism, improve crop yield and mitigate global climate change. |
| 英文关键词 | Red-type Rubisco; Heterologous assembly; Expression; Chaperonin; Red algae; Diatom |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:001223147400001 |
| 来源期刊 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/293420 |
| 作者单位 | East China University of Science & Technology; East China University of Science & Technology; Shihezi University |
| 推荐引用方式 GB/T 7714 | . Heterologous assembly of red-type Rubisco and functional identification of chaperonin in Porphyridium purpureum[J],2024,79. |
| APA | (2024).Heterologous assembly of red-type Rubisco and functional identification of chaperonin in Porphyridium purpureum.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,79. |
| MLA | "Heterologous assembly of red-type Rubisco and functional identification of chaperonin in Porphyridium purpureum".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 79(2024). |
| 条目包含的文件 | 条目无相关文件。 | |||||
| 个性服务 |
| 推荐该条目 |
| 保存到收藏夹 |
| 导出为Endnote文件 |
| 谷歌学术 |
| 谷歌学术中相似的文章 |
| 百度学术 |
| 百度学术中相似的文章 |
| 必应学术 |
| 必应学术中相似的文章 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
