气候变化领域集成服务门户(CCPortal): Uncovering the Dynamics of Urease and Carbonic Anhydrase Genes in Ureolysis, Carbon Dioxide Hydration, and Calcium Carbonate Precipitation

CCPortal

DOI	10.1021/acs.est.3c06617
	Uncovering the Dynamics of Urease and Carbonic Anhydrase Genes in Ureolysis, Carbon Dioxide Hydration, and Calcium Carbonate Precipitation
	Saracho, Alexandra Clara; Marek, Ewa J.
发表日期	2024
ISSN	0013-936X
EISSN	1520-5851
起始页码	58
结束页码	2
卷号	58 期号:2
英文摘要	The hydration of CO2 suffers from kinetic inefficiencies that make its natural trapping impractically sluggish. However, CO2-fixing carbonic anhydrases (CAs) remarkably accelerate its equilibration by 6 orders of magnitude and are, therefore, ideal catalysts. Notably, CA has been detected in ureolytic bacteria, suggesting its potential involvement in microbially induced carbonate precipitation (MICP), yet the dynamics of the urease (Ur) and CA genes remain poorly understood. Here, through the use of the ureolytic bacteriumSporosarcina pasteurii, we investigate the differing role of Ur and CA in ureolysis, CO2 hydration, and CaCO3 precipitation with increasing CO2(g) concentrations. We show that Ur gene up-regulation coincides with an increase in [HCO3-] following the hydration of CO2 to HCO3- by CA. Hence, CA physiologically promotes buffering, which enhances solubility trapping and affects the phase of the CaCO3 mineral formed. Understanding the role of CO2 hydration on the performance of ureolysis and CaCO3 precipitation provides essential new insights, required for the development of next-generation biocatalyzed CO2 trapping technologies.
英文关键词	climate change; CO2 sequestration; microbially induced calcium carbonate precipitation; microbialmetabolism
语种	英语
WOS研究方向	Engineering ; Environmental Sciences & Ecology
WOS类目	Engineering, Environmental ; Environmental Sciences
WOS记录号	WOS:001144008100001
来源期刊	ENVIRONMENTAL SCIENCE & TECHNOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/293320
作者单位	University of Texas System; University of Texas Austin; University of Cambridge
推荐引用方式 GB/T 7714	Saracho, Alexandra Clara,Marek, Ewa J.. Uncovering the Dynamics of Urease and Carbonic Anhydrase Genes in Ureolysis, Carbon Dioxide Hydration, and Calcium Carbonate Precipitation[J],2024,58(2).
APA	Saracho, Alexandra Clara,&Marek, Ewa J..(2024).Uncovering the Dynamics of Urease and Carbonic Anhydrase Genes in Ureolysis, Carbon Dioxide Hydration, and Calcium Carbonate Precipitation.ENVIRONMENTAL SCIENCE & TECHNOLOGY,58(2).
MLA	Saracho, Alexandra Clara,et al."Uncovering the Dynamics of Urease and Carbonic Anhydrase Genes in Ureolysis, Carbon Dioxide Hydration, and Calcium Carbonate Precipitation".ENVIRONMENTAL SCIENCE & TECHNOLOGY 58.2(2024).