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| DOI | 10.1111/ppa.13911 |
| Development of a one-step multiplex reverse transcription-polymerase chain reaction and Luminex xTAG assay for the simultaneous detection of yellowing viruses infecting sugar beet | |
| 发表日期 | 2024 |
| ISSN | 0032-0862 |
| EISSN | 1365-3059 |
| 英文摘要 | Yellowing viruses are an increasing threat to sugar beet cultivation, due to limitations on insecticide usage and climate change. Virus detection, monitoring and resistance breeding are key to secure high sugar beet yields in the future. For this research, a one-step multiplex reverse transcription (mRT)-PCR method was designed to detect simultaneously beet mild yellowing virus, beet chlorosis virus, beet yellows virus (BYV), beet mosaic virus and turnip yellows virus. The addition of Luminex xTAG array technology was used as a follow-up method to increase assay specificity. The one-step mRT-PCR was evaluated on 22 field samples with single and mixed virus infections. The xTAG assay works as expected both in a simplex and multiplex setting, except that BYV detection needs optimization in the multiplex setting. In the future, the Luminex xTAG assay would be an excellent method for the detection of beet yellowing viruses due to its high specificity and the potential to increase the number of targets. |
| 英文关键词 | beet yellowing viruses; Luminex assay; methods; multiplex PCR; sugar beet |
| 语种 | 英语 |
| WOS研究方向 | Agriculture ; Plant Sciences |
| WOS类目 | Agronomy ; Plant Sciences |
| WOS记录号 | WOS:001208285000001 |
| 来源期刊 | PLANT PATHOLOGY
 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://gcip.llas.ac.cn/handle/2XKMVOVA/293223 |
| 作者单位 | Wageningen University & Research; Wageningen University & Research |
| 推荐引用方式 GB/T 7714 | . Development of a one-step multiplex reverse transcription-polymerase chain reaction and Luminex xTAG assay for the simultaneous detection of yellowing viruses infecting sugar beet[J],2024. |
| APA | (2024).Development of a one-step multiplex reverse transcription-polymerase chain reaction and Luminex xTAG assay for the simultaneous detection of yellowing viruses infecting sugar beet.PLANT PATHOLOGY. |
| MLA | "Development of a one-step multiplex reverse transcription-polymerase chain reaction and Luminex xTAG assay for the simultaneous detection of yellowing viruses infecting sugar beet".PLANT PATHOLOGY (2024). |
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