气候变化领域集成服务门户(CCPortal): Effect of heat stress on the hypothalamic expression profile of water homeostasis-associated genes in low- and high-water efficient chicken lines

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DOI	10.14814/phy2.15972
	Effect of heat stress on the hypothalamic expression profile of water homeostasis-associated genes in low- and high-water efficient chicken lines
	Aloui, Loujain; Greene, Elizabeth S.; Tabler, Travis; Lassiter, Kentu; Thompson, Kevin; Bottje, Walter G.; Orlowski, Sara; Dridi, Sami
发表日期	2024
ISSN	2051-817X
起始页码	12
结束页码	5
卷号	12 期号:5
英文摘要	With climate change, selection for water efficiency and heat resilience are vitally important. We undertook this study to determine the effect of chronic cyclic heat stress (HS) on the hypothalamic expression profile of water homeostasis-associated markers in high (HWE)- and low (LWE)-water efficient chicken lines. HS significantly elevated core body temperatures of both lines. However, the amplitude was higher by 0.5-1 degrees C in HWE compared to their LWE counterparts. HWE line drank significantly less water than LWE during both thermoneutral (TN) and HS conditions, and HS increased water intake in both lines with pronounced magnitude in LWE birds. HWE had better feed conversion ratio (FCR), water conversion ratio (WCR), and water to feed intake ratio. At the molecular level, the overall hypothalamic expression of aquaporins (AQP8 and AQP12), arginine vasopressin (AVP) and its related receptor AVP2R, angiotensinogen (AGT), angiotensin II receptor type 1 (AT1), and calbindin 2 (CALB2) were significantly lower; however, CALB1 mRNA and AQP2 protein levels were higher in HWE compared to LWE line. Compared to TN conditions, HS exposure significantly increased mRNA abundances of AQPs (8, 12), AVPR1a, natriuretic peptide A (NPPA), angiotensin I-converting enzyme (ACE), CALB1 and 2, and transient receptor potential cation channel subfamily V member 1 and 4 (TRPV1 and TRPV4) as well as the protein levels of AQP2, however it decreased that of AQP4 gene expression. A significant line by environment interaction was observed in several hypothalamic genes. Heat stress significantly upregulated AQP2 and SCT at mRNA levels and AQP1 and AQP3 at both mRNA and protein levels, but it downregulated that of AQP4 protein only in LWE birds. In HWE broilers, however, HS upregulated the hypothalamic expression of renin (REN) and AVPR1b genes and AQP5 proteins, but it downregulated that of AQP3 protein. The hypothalamic expression of AQP (5, 7, 10, and 11) genes was increased by HS in both chicken lines. In summary, this is the first report showing improvement of growth performances in HWE birds. The hypothalamic expression of several genes was affected in a line- and/or environment-dependent manner, revealing potential molecular signatures for water efficiency and/or heat tolerance in chickens. imageDivergently selected broilers for high water efficiency drunk less water during high environmental temperature and perform better than their low-water efficient counterparts through modulation of hypothalamic AVP, RAAS, and aquaporin systems.
英文关键词	broilers; gene expression; growth; hypothalamus; selection; water efficiency
语种	英语
WOS研究方向	Physiology
WOS类目	Physiology
WOS记录号	WOS:001182020000001
来源期刊	PHYSIOLOGICAL REPORTS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/293057
作者单位	University of Arkansas System; University of Arkansas Fayetteville; Universite de Carthage; University of Arkansas System; University of Arkansas Fayetteville; University of Arkansas System; University of Arkansas Fayetteville
推荐引用方式 GB/T 7714	Aloui, Loujain,Greene, Elizabeth S.,Tabler, Travis,et al. Effect of heat stress on the hypothalamic expression profile of water homeostasis-associated genes in low- and high-water efficient chicken lines[J],2024,12(5).
APA	Aloui, Loujain.,Greene, Elizabeth S..,Tabler, Travis.,Lassiter, Kentu.,Thompson, Kevin.,...&Dridi, Sami.(2024).Effect of heat stress on the hypothalamic expression profile of water homeostasis-associated genes in low- and high-water efficient chicken lines.PHYSIOLOGICAL REPORTS,12(5).
MLA	Aloui, Loujain,et al."Effect of heat stress on the hypothalamic expression profile of water homeostasis-associated genes in low- and high-water efficient chicken lines".PHYSIOLOGICAL REPORTS 12.5(2024).