气候变化领域集成服务门户(CCPortal): Comparative transcriptomic analysis delineates adaptation strategies of Rana kukunoris toward cold stress on the Qinghai-Tibet Plateau

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DOI	10.1186/s12864-024-10248-8
	Comparative transcriptomic analysis delineates adaptation strategies of Rana kukunoris toward cold stress on the Qinghai-Tibet Plateau
	Zhang, Tao; Jia, Lun; Niu, Zhiyi; Li, Xinying; Men, Shengkang; Jiang, Lu; Ma, Miaojun; Wang, Huihui; Tang, Xiaolong; Chen, Qiang
发表日期	2024
ISSN	1471-2164
起始页码	25
结束页码	1
卷号	25 期号:1
英文摘要	Background Cold hardiness is fundamental for amphibians to survive during the extremely cold winter on the Qinghai-Tibet plateau. Exploring the gene regulation mechanism of freezing-tolerant Rana kukunoris could help us to understand how the frogs survive in winter. Results Transcriptome of liver and muscle of R. kukunoris collected in hibernation and spring were assisted by single molecule real-time (SMRT) sequencing technology. A total of 10,062 unigenes of R. kukunoris were obtained, and 9,924 coding sequences (CDS) were successfully annotated. Our examination of the mRNA response to whole body freezing and recover in the frogs revealed key genes concerning underlying antifreeze proteins and cryoprotectants (glucose and urea). Functional pathway analyses revealed differential regulated pathways of ribosome, energy supply, and protein metabolism which displayed a freeze-induced response and damage recover. Genes related to energy supply in the muscle of winter frogs were up-regulated compared with the muscle of spring frogs. The liver of hibernating frogs maintained modest levels of protein synthesis in the winter. In contrast, the liver underwent intensive high levels of protein synthesis and lipid catabolism to produce substantial quantity of fresh proteins and energy in spring. Differences between hibernation and spring were smaller than that between tissues, yet the physiological traits of hibernation were nevertheless passed down to active state in spring. Conclusions Based on our comparative transcriptomic analyses, we revealed the likely adaptive mechanisms of R. kukunoris. Ultimately, our study expands genetic resources for the freezing-tolerant frogs.
英文关键词	Pacbio sequel; Freeze exposure; Hibernation; Emergence period; Liver; Muscle; Tissue specificity
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:001201821500003
来源期刊	BMC GENOMICS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/289758
作者单位	Lanzhou University; Lanzhou University; Lanzhou University
推荐引用方式 GB/T 7714	Zhang, Tao,Jia, Lun,Niu, Zhiyi,et al. Comparative transcriptomic analysis delineates adaptation strategies of Rana kukunoris toward cold stress on the Qinghai-Tibet Plateau[J],2024,25(1).
APA	Zhang, Tao.,Jia, Lun.,Niu, Zhiyi.,Li, Xinying.,Men, Shengkang.,...&Chen, Qiang.(2024).Comparative transcriptomic analysis delineates adaptation strategies of Rana kukunoris toward cold stress on the Qinghai-Tibet Plateau.BMC GENOMICS,25(1).
MLA	Zhang, Tao,et al."Comparative transcriptomic analysis delineates adaptation strategies of Rana kukunoris toward cold stress on the Qinghai-Tibet Plateau".BMC GENOMICS 25.1(2024).