气候变化领域集成服务门户(CCPortal): Temperature and immune challenges modulate the transcription of genes of the ubiquitin and apoptosis pathways in two high-latitude Notothenioid fish across the Antarctic Polar Front

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DOI	10.1007/s10695-024-01348-z
	Temperature and immune challenges modulate the transcription of genes of the ubiquitin and apoptosis pathways in two high-latitude Notothenioid fish across the Antarctic Polar Front
	Saravia, Julia; Nualart, Daniela; Paschke, Kurt; Pontigo, Juan Pablo; Navarro, Jorge M.; Vargas-Chacoff, Luis
发表日期	2024
ISSN	0920-1742
EISSN	1573-5168
英文摘要	Thermal variations due to global climate change are expected to modify the distributions of marine ectotherms, with potential pathogen translocations. This is of particular concern at high latitudes where cold-adapted stenothermal fish such as the Notothenioids occur. However, little is known about the combined effects of thermal fluctuations and immune challenges on the balance between cell damage and repair processes in these fish. The aim of this study was to determine the effect of thermal variation on specific genes involved in the ubiquitination and apoptosis pathways in two congeneric Notothenioid species, subjected to simulated bacterial and viral infections. Adult fish of Harpagifer bispinis and Harpagifer antarcticus were collected from Punta Arenas (Chile) and King George Island (Antarctica), respectively, and distributed as follows: injected with PBS (control), LPS (2.5 mg/kg) or Poly I:C (2 mg/kg) and then submitted to 2, 5 and 8 degrees C. After 1 week, samples of gills, liver and spleen were taken to evaluate the expression by real-time PCR of specific genes involved in ubiquitination (E3-ligase enzyme) and apoptosis (BAX and SMAC/DIABLO). Gene expression was tissue-dependent and increased with increasing temperature in the gills and liver while showing an opposite pattern in the spleen. Studying a pair of sister species that occur across the Antarctic Polar Front can help us understand the particular pressures of intertidal lifestyles and the effect of temperature in combination with biological stressors on cell damage and repair capacity in a changing environment.
英文关键词	Antarctic; Subantarctic; Fish; Gene expression; Programmed cell death
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Fisheries ; Physiology
WOS类目	Biochemistry & Molecular Biology ; Fisheries ; Physiology
WOS记录号	WOS:001207644500002
来源期刊	FISH PHYSIOLOGY AND BIOCHEMISTRY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/289642
作者单位	Universidad Austral de Chile; Universidad Austral de Chile; Universidad Austral de Chile; Universidad Austral de Chile; Universidad Austral de Chile; Universidad Austral de Chile; Universidad San Sebastian
推荐引用方式 GB/T 7714	Saravia, Julia,Nualart, Daniela,Paschke, Kurt,et al. Temperature and immune challenges modulate the transcription of genes of the ubiquitin and apoptosis pathways in two high-latitude Notothenioid fish across the Antarctic Polar Front[J],2024.
APA	Saravia, Julia,Nualart, Daniela,Paschke, Kurt,Pontigo, Juan Pablo,Navarro, Jorge M.,&Vargas-Chacoff, Luis.(2024).Temperature and immune challenges modulate the transcription of genes of the ubiquitin and apoptosis pathways in two high-latitude Notothenioid fish across the Antarctic Polar Front.FISH PHYSIOLOGY AND BIOCHEMISTRY.
MLA	Saravia, Julia,et al."Temperature and immune challenges modulate the transcription of genes of the ubiquitin and apoptosis pathways in two high-latitude Notothenioid fish across the Antarctic Polar Front".FISH PHYSIOLOGY AND BIOCHEMISTRY (2024).