气候变化领域集成服务门户(CCPortal): Identification, Nutrient Composition, and Evaluation of a Wild Pleurotus citrinopileatus Strain (X21156) from Tibet for Antioxidant and Cytotoxic Activities

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DOI	10.3390/horticulturae10040377
	Identification, Nutrient Composition, and Evaluation of a Wild Pleurotus citrinopileatus Strain (X21156) from Tibet for Antioxidant and Cytotoxic Activities
	Xiao, Xiaoshan; Li, Yun; Li, Xiaomin; Hu, Xin; Zhang, Junli; Wu, Xiaoping; Fu, Junsheng
发表日期	2024
EISSN	2311-7524
起始页码	10
结束页码	4
卷号	10 期号:4
英文摘要	A fungal strain (X21156) collected in Tibet was used as the material, identified based on its morphological characteristics and internal transcribed spacer (ITS) sequence; its optimal culture conditions were analyzed by single-factor experiments; artificial domestication and cultivation were carried out; its nutrient composition was determined; and the bioactivities of its polysaccharides were detected using chemical antioxidant assays and MTT assays. The results showed that the strain was Pleurotus citrinopileatus Sing. Its optimal culture conditions were a pH of 7, a temperature of 25 degrees C, glucose (20 gL-1) as the carbon source, and yeast powder (20 gL-1) as the nitrogen source. The fresh weight of a single domesticated fruiting body was 41.16 g. The strain had high protein (28.5%), high fiber (34%), and low fat (1.4%) contents, with high proportions of fresh and sweet amino acids. Polysaccharides had good scavenging ability on ABTS+, DPPH, and OH free radicals (EC50 0.06 mg/mL, 1.21 mg/mL, and 3.62 mg/mL, respectively), and the cytotoxicity of polysaccharides to hepatocellular carcinoma cells (HepG2) (IC50: 1.69 mg/mL) was higher than that of triple-negative breast cancer cells (MDA-MB-468) (IC50: 1.76 mg/mL). In conclusion, the study provides a reference on the optimal culture conditions, domestication and cultivation, and dietary and medicinal values of wild P. citrinopileatus Sing.
英文关键词	Pleurotus citrinopileatus; fungi identification; biological properties; nutritional composition; antioxidant activities; cytotoxic effect
语种	英语
WOS研究方向	Agriculture
WOS类目	Horticulture
WOS记录号	WOS:001211285900001
来源期刊	HORTICULTURAE
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/288583
作者单位	Fujian Agriculture & Forestry University; Tibet Academy of Agriculture & Animal Husbandry Sciences; Fujian Agriculture & Forestry University
推荐引用方式 GB/T 7714	Xiao, Xiaoshan,Li, Yun,Li, Xiaomin,et al. Identification, Nutrient Composition, and Evaluation of a Wild Pleurotus citrinopileatus Strain (X21156) from Tibet for Antioxidant and Cytotoxic Activities[J],2024,10(4).
APA	Xiao, Xiaoshan.,Li, Yun.,Li, Xiaomin.,Hu, Xin.,Zhang, Junli.,...&Fu, Junsheng.(2024).Identification, Nutrient Composition, and Evaluation of a Wild Pleurotus citrinopileatus Strain (X21156) from Tibet for Antioxidant and Cytotoxic Activities.HORTICULTURAE,10(4).
MLA	Xiao, Xiaoshan,et al."Identification, Nutrient Composition, and Evaluation of a Wild Pleurotus citrinopileatus Strain (X21156) from Tibet for Antioxidant and Cytotoxic Activities".HORTICULTURAE 10.4(2024).