气候变化领域集成服务门户(CCPortal): Genome and evolution of Tibet orbivirus, TIBOV (genus Orbivirus, family Reoviridae)

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DOI	10.3389/fcimb.2024.1327780
	Genome and evolution of Tibet orbivirus, TIBOV (genus Orbivirus, family Reoviridae)
	Gao, Tingting; Li, Minghua; Liu, Hong; Fu, Shihong; Wang, Huanyu; Liang, Guodong
发表日期	2024
ISSN	2235-2988
起始页码	14
卷号	14
英文摘要	Tibet orbivirus (TIBOV) was first isolated from Anopheles maculatus mosquitoes in Xizang, China, in 2009. In recent years, more TIBOV strains have been isolated in several provinces across China, Japan, East Asia, and Nepal, South Asia. Furthermore, TIBOVs have also been isolated from Culex mosquitoes, and several midge species. Additionally, TIBOV neutralizing antibodies have been detected in serum specimens from several mammals, including cattle, sheep, and pigs. All of the evidence suggests that the geographical distribution of TIBOVs has significantly expanded in recent years, with an increased number of vector species involved in its transmission. Moreover, the virus demonstrated infectivity towards a variety of animals. Although TIBOV is considered an emerging orbivirus, detailed reports on its genome and molecular evolution are currently lacking. Thus, this study performed the whole-genome nucleotide sequencing of three TIBOV isolates from mosquitoes and midges collected in China in 2009, 2011, and 2019. Furthermore, the genome and molecular genetic evolution of TIBOVs isolated from different countries, periods, and hosts (mosquitoes, midges, and cattle) was systematically analyzed. The results revealed no molecular specificity among TIBOVs isolated from different countries, periods, and vectors. Meanwhile, the time-scaled phylogenetic analysis demonstrated that the most recent common ancestor (TMRCA) of TIBOV appeared approximately 797 years ago (95% HPD: 16-2347) and subsequently differentiated at least three times, resulting in three distinct genotypes. The evolutionary rate of TIBOVs was about 2.12 x 10(-3) nucleotide substitutions per site per year (s/s/y) (95% HPD: 3.07 x 10(-5), 9.63 x 10(-3)), which is similar to that of the bluetongue virus (BTV), also in the Orbivirus genus. Structural analyses of the viral proteins revealed that the three-dimensional structures of the outer capsid proteins of TIBOV and BTV were similar. These results suggest that TIBOV is a newly discovered and rapidly evolving virus transmitted by various blood-sucking insects. Given the potential public health burden of this virus and its high infectious rate in a wide range of animals, it is significant to strengthen research on the genetic variation of TIBOVs in blood-feeding insects and mammals in the natural environment and the infection status in animals.
英文关键词	Tibet orbivirus; Reoviridae; Orbivirus; viral genome structure; molecular genetics; phylogeny
语种	英语
WOS研究方向	Immunology ; Microbiology
WOS类目	Immunology ; Microbiology
WOS记录号	WOS:001187225800001
来源期刊	FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/288180
作者单位	Shandong University of Technology; Chinese Center for Disease Control & Prevention; National Institute for Viral Disease Control & Prevention, Chinese Center for Disease Control & Prevention
推荐引用方式 GB/T 7714	Gao, Tingting,Li, Minghua,Liu, Hong,et al. Genome and evolution of Tibet orbivirus, TIBOV (genus Orbivirus, family Reoviridae)[J],2024,14.
APA	Gao, Tingting,Li, Minghua,Liu, Hong,Fu, Shihong,Wang, Huanyu,&Liang, Guodong.(2024).Genome and evolution of Tibet orbivirus, TIBOV (genus Orbivirus, family Reoviridae).FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,14.
MLA	Gao, Tingting,et al."Genome and evolution of Tibet orbivirus, TIBOV (genus Orbivirus, family Reoviridae)".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 14(2024).