气候变化领域集成服务门户(CCPortal): Bioprospecting of Actinobacterial Diversity and Antibacterial Secondary Metabolites from the Sediments of Four Saline Lakes on the Northern Tibetan Plateau

CCPortal

DOI	10.3390/microorganisms11102475
	Bioprospecting of Actinobacterial Diversity and Antibacterial Secondary Metabolites from the Sediments of Four Saline Lakes on the Northern Tibetan Plateau
	Liu, Shao-Wei; Zhai, Xiao-Xu; Liu, Di; Liu, Yu-Yu; Sui, Li-Ying; Luo, Ke-Ke; Yang, Qin; Li, Fei-Na; Nikandrova, Arina A.; Imamutdinova, Arina N.; Lukianov, Dmitrii A.; Osterman, Ilya A.; Sergiev, Petr V.; Zhang, Ben-Yin; Zhang, De-Jun; Xue, Chun-Mei; Sun, Cheng-Hang
发表日期	2023
EISSN	2076-2607
卷号	11 期号:10
英文摘要	The Tibetan Plateau, known as the Roof of the World and The Third Pole, harbors numerous saline lakes primarily distributed in the Northern Tibetan Plateau. However, the challenging conditions of high altitude, low oxygen level, and harsh climate have limited investigations into the actinobacteria from these saline lakes. This study focuses on investigating the biodiversity and bioactive secondary metabolites of cultivable actinobacteria isolated from the sediments of four saline lakes on the Northern Tibetan Plateau. A total of 255 actinobacterial strains affiliated with 21 genera in 12 families of 7 orders were recovered by using the pure culture technique and 16S rRNA gene phylogenetic analysis. To facilitate a high-throughput bioactivity evaluation, 192 isolates underwent OSMAC cultivation in a miniaturized 24-well microbioreactor system (MATRIX cultivation). The antibacterial activity of crude extracts was then evaluated in a 96-well plate antibacterial assay. Forty-six strains demonstrated antagonistic effects against at least one tested pathogen, and their underlying antibacterial mechanisms were further investigated through a dual-fluorescent reporter assay (pDualrep2). Two Streptomyces strains (378 and 549) that produce compounds triggering DNA damage were prioritized for subsequent chemical investigations. Metabolomics profiling involving HPLC-UV/vis, UPLC-QTOF-MS/MS, and molecular networking identified three types of bioactive metabolites belonging to the aromatic polyketide family, i.e., cosmomycin, kidamycin, and hedamycin. In-depth analysis of the metabolomic data unveiled some potentially novel anthracycline compounds. A genome mining study based on the whole-genome sequences of strains 378 and 549 identified gene clusters potentially responsible for cosmomycin and kidamycin biosynthesis. This work highlights the effectiveness of combining metabolomic and genomic approaches to rapidly identify bioactive chemicals within microbial extracts. The saline lakes on the Northern Tibetan Plateau present prospective sources for discovering novel actinobacteria and biologically active compounds.
关键词	saline lakes actinobacteria Northern Tibetan Plateau antibacterial metabolites MATRIX cultivation molecular networking
英文关键词	NATURAL-PRODUCTS; COSMOMYCIN-D; ANTHRACYCLINE ANTIBIOTICS; SALT-LAKE; GEN. NOV.; ANTITUMOR ANTIBIOTICS; COMMUNITY STRUCTURE; MASS-SPECTROMETRY; SP. NOV.; DNA
WOS研究方向	Microbiology
WOS记录号	WOS:001095553200001
来源期刊	MICROORGANISMS
文献类型	期刊论文
条目标识符	http://gcip.llas.ac.cn/handle/2XKMVOVA/283838
作者单位	Chinese Academy of Medical Sciences - Peking Union Medical College; Peking Union Medical College; Qinghai University; Jiamusi University; Tianjin University Science & Technology; Capital Medical University; Skolkovo Institute of Science & Technology; Lomonosov Moscow State University; Lomonosov Moscow State University; Qinghai University
推荐引用方式 GB/T 7714	Liu, Shao-Wei,Zhai, Xiao-Xu,Liu, Di,et al. Bioprospecting of Actinobacterial Diversity and Antibacterial Secondary Metabolites from the Sediments of Four Saline Lakes on the Northern Tibetan Plateau[J],2023,11(10).
APA	Liu, Shao-Wei.,Zhai, Xiao-Xu.,Liu, Di.,Liu, Yu-Yu.,Sui, Li-Ying.,...&Sun, Cheng-Hang.(2023).Bioprospecting of Actinobacterial Diversity and Antibacterial Secondary Metabolites from the Sediments of Four Saline Lakes on the Northern Tibetan Plateau.MICROORGANISMS,11(10).
MLA	Liu, Shao-Wei,et al."Bioprospecting of Actinobacterial Diversity and Antibacterial Secondary Metabolites from the Sediments of Four Saline Lakes on the Northern Tibetan Plateau".MICROORGANISMS 11.10(2023).